Survival of granulocytic progenitors in the nonadherent and adherent compartments of human long-term marrow cultures

F. T. Slovick, C. N. Abboud, J. K. Brennan, M. A. Lichtman

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36 Scopus citations

Abstract

We have studied the survival of granulocytic and monocytic progenitor cells (CFU-GM) in human liquid marrow cultures. CFU-GM were present in the nonadherent cell population for a mean of 12 weeks without recharging. Histochemical analysis of agar gels revealed that most day-7 colonies were of neutrophilic type (CFU-N), whereas the majority of day-14 colonies were of mixed neutrophilic-macrophagic type (CFU-NM) for the first four weeks of culture and became predominantly of macrophagic type (CFU-M) thereafter. Eosinophilic colonies (CFU-Eo) declined after week 2 of culture. We have documented that CFU-GM were present in the adherent layer of these cultures, and that the CFU-GM in the nonadherent compartment arise from the adherent layer. In addition, we have compared the pre-CFU-GM survival in the adherent and nonadherent populations and determined that these progenitors were rapidly depleted from both compartments though their survival at the end of week 1 was better in the adherent than in the nonadherent layer.

Original languageEnglish
Pages (from-to)327-338
Number of pages12
JournalExperimental Hematology
Volume12
Issue number5
StatePublished - Jan 1 1984
Externally publishedYes

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