Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages

Deirdre M. O'Riordan, Joseph E. Standing, Kun Young Kwon, Donald Chang, Edmond C. Crouch, Andrew H. Limper

Research output: Contribution to journalArticle

144 Scopus citations

Abstract

Pneumocystis carinii interacts with glycoproteins present in the lower respiratory tract through its mannose-rich surface antigen complex termed gpA. Surfactant protein D (SP-D) is a recently described component of the airspace lining material that possesses a calcium-dependent lectin domain capable of interacting with glycoconjugates present on microorganisms and leukocytes. Accordingly, we evaluated the extent and localization of SP-D in the lower respiratory tract during Pneumocystis pneumonia in an immunosuppressed rat model and examined its role in modulating interaction of P. carinii with macrophages. We report that SP-D is a major component of the alveolar exudates that typify P. carinii pneumonia and is present bound to the surface of P. carinii organisms in vivo. We further demonstrate that SP- D binds to P. carinii through saccharide-mediated interactions with gpA present on the surface of the organism. Lastly, we show that SP-D augments binding of P. carinii to alveolar macrophages, but does not significantly enhance macrophage phagocytosis of the organism. The interaction of SP-D with gpA represents an additional important component of the host-parasite relationship during P. carinii pneumonia.

Original languageEnglish
Pages (from-to)2699-2710
Number of pages12
JournalJournal of Clinical Investigation
Volume95
Issue number6
DOIs
StatePublished - Jun 1995

Keywords

  • Pneumocystis carinii
  • gpA
  • lectin
  • macrophages
  • surfactant protein D

Fingerprint Dive into the research topics of 'Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages'. Together they form a unique fingerprint.

  • Cite this