Surfactant protein-D enhances phagocytosis and pulmonary clearance of respiratory syncytial virus

Ann Marie LeVine, James Elliott, Jeffrey A. Whitsett, Anon Srikiatkhachorn, Erika Crouch, Nihal DeSilva, Thomas Korfhagen

Research output: Contribution to journalArticlepeer-review

132 Scopus citations


Surfactant protein (SP)-D gene targeted (SP-D-/-) and wild-type mice were infected with respiratory syncytial virus (RSV) by intratracheal instillation. Decreased clearance of RSV was observed in SP-D-/- mice. Deficiency of SP-D was associated with increased inflammation and inflammatory cell recruitment in the lung after infection. In vitro, SP-D bound RSV-infected Vero cells. Binding was inhibited with ethylenediamine tetraacetic acid and maltose, suggesting that the carbohydrate recognition domain of SP-D recognizes RSV glycoproteins in a calcium-dependent manner. SP-D bound specifically to the RSV proteins C and F. Phagocytosis of RSV by alveolar macrophages was reduced in the absence of SP-D in vivo, and SP-D enhanced phagocytosis of RSV by alveolar macrophages and neutrophils but not peritoneal macrophages in vitro. Oxygen radical production by alveolar macrophages from SP-D+/+ and SP-D+/+ mice was decreased after RSV infection, and SP-D ameliorated the inhibitory effects of RSV on oxygen radical production by macrophages and neutrophils in vitro. Because the airway is the usual portal of entry for RSV and other respiratory pathogens, the local production of SP-D is likely to play a role in innate defense responses to inhaled viruses.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Issue number2 I
StatePublished - Aug 2004


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