Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation

Delphine C. Malherbe, Veit J. Erpenbeck, Soman N. Abraham, Erika C. Crouch, Jens M. Hohlfeld, Jo Rae Wright

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Mast cells play a key role in allergy and asthma. They reside at the host-environment interface and are among the first cells to make contact with inhaled microorganisms and particulate antigens. Pulmonary surfactant proteins A and D (SP-A and SP-D) function in lung host defense by enhancing microbe phagocytosis and mediating other immune cell functions, but little is known about their effects on mast cells. We hypothesized that SP-A and/or SP-D modulate IgE-dependent mast cell functions. Pollen starch granules (PSG) extracted from Dactylis glomerata and coated with trinitrophenol (TNP) were used as a model of an inhaled organic particulate allergen. Our data revealed that SP-D inhibited by 50% the release of β-hexosaminidase by peritoneal mast cells sensitized with IgE anti-TNP and stimulated with TNP-PSG. In contrast, SP-A had no effect. Furthermore, SP-D aggregated PSG in a dose-dependent manner, and this aggregation was mediated by SP-D's carbohydrate recognition domain. A single arm SP-D mutant (RrSP-Dser15,20) neither aggregated PSG nor inhibited degranulation, suggesting that multimerization of SP-D is required for maximal PSG aggregation and inhibition of PSG-induced mast cell degranulation. This study is the first to demonstrate that SP-D modulates IgE-mediated mast cell functions, which are important in asthma and allergic inflammation.

Original languageEnglish
Pages (from-to)L856-L866
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume289
Issue number5 33-5
DOIs
StatePublished - Nov 2005

Keywords

  • Allergen
  • Collectins
  • Immunoglobulin E
  • Innate immunity
  • Lung

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