Surfactant protein d-coated klebsiella pneumoniae stimulatescytokine production in mononuclear phagocytes

Yona Keisari, Hongbin Wang, Adi Mesika, Robert Matatov, Liat Nissimov, Erika Crouch, Itzhak Ofek

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17 Scopus citations

Abstract

Encapsulated Klebsiella pneumoniae strains K21a, K10, and K50, all of which contain dimannose sequences in their capsular polysaccharides that are recognized by the mannose receptor of macrophages, stimulated interleukin secretion and cytokine mRNA expression by human monocyte-derived macrophages. By contrast, the corresponding unencapsulated phase variants and the K2 strain, which lack the dimannose sequence, did not. Coating of unencapsulated phase variants of Klebsiella strains with surfactant protein (SP)-D resulted in marked stimulation of cytokine mRNA accumulation. The induction of cytokine mRNA via the mannose receptor occurred only in monocyte-derived macrophages, whereas that caused by SP-D-coated Klebsiella strains occurred in both macrophages and peripheral-blood monocytes. The results suggested that innate immunity against pulmonary pathogens might be mediated by SP-D, which acts as an opsonin to enhance the interaction of macrophages with unencapsulated phase variants originating from the upper respiratory tract, and by macrophage mannose receptors, which recognize encapsulated variants expressing capsular dimannose residues.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalJournal of Leukocyte Biology
Volume70
Issue number1
StatePublished - Jul 16 2001

Keywords

  • Capsular polysaccharides
  • Capsule
  • Human
  • Macrophages
  • Mannose receptor
  • Monocytes

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    Keisari, Y., Wang, H., Mesika, A., Matatov, R., Nissimov, L., Crouch, E., & Ofek, I. (2001). Surfactant protein d-coated klebsiella pneumoniae stimulatescytokine production in mononuclear phagocytes. Journal of Leukocyte Biology, 70(1), 135-141.