Surface IgM mediated regulation of RAG gene expression in Eμ-N-myc B cell lines

A. Ma, P. Fisher, R. Dildrop, E. Oltz, G. Rathbun, P. Achacoso, A. Stall, F. W. Alt

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Abstract

Transgenic mice carrying either the c-myc or N-myc oncogene deregulated by the immunoglobulin heavy chain enhancer element (Eμ) develop both pre-B and B cell lymphomas (Eμ-c-myc and Eμ-N-myc lymphomas). We report here that B cell lines derived from these tumors, as well as a line derived from v-myc retroviral transformation, simultaneously express surface immunoglobulin (a hallmark of mature B cells) as well as a common subset of genes normally restricted to the pre-B stage of development including the recombinase activating genes RAG-1 and RAG-2. Continued RAG-1 and RAG-2 expression in these lines is associated with VDJ recombinase activity detected with a VDJ recombination substrate. Cross-linking of the surface immunoglobulin on these lines with an anti-μ antibody leads to rapid, specific and reversible down-regulation of RAG-I and RAG-2 gene expression. We also find that a small but significant percentage of normal surface immunoglobulin bearing bone marrow B cells express the RAG-I gene. These findings are discussed in the context of their possible implications for the control of specific gene expression during the pre-B to B cell transition.

Original languageEnglish
Pages (from-to)2727-2734
Number of pages8
JournalEMBO Journal
Volume11
Issue number7
StatePublished - Jan 1 1992

Keywords

  • Down-regulation
  • Eμ-N-myc B cells
  • Pre-B genes
  • Recombinase activating genes

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    Ma, A., Fisher, P., Dildrop, R., Oltz, E., Rathbun, G., Achacoso, P., Stall, A., & Alt, F. W. (1992). Surface IgM mediated regulation of RAG gene expression in Eμ-N-myc B cell lines. EMBO Journal, 11(7), 2727-2734.