TY - JOUR
T1 - Suppressor of RNA silencing encoded by Beet yellows virus
AU - Reed, Jonathan C.
AU - Kasschau, Kristin D.
AU - Prokhnevsky, Alexey I.
AU - Gopinath, Kodetham
AU - Pogue, Gregory P.
AU - Carrington, James C.
AU - Dolja, Valerian V.
N1 - Funding Information:
This work was supported by grants from the U.S. Department of Agriculture (NRICGP 2001-35319-10875 to V.V.D. and 2002-35319-11560 to J.C.C.), National Institutes of Health (R1GM53190B), and Large Scale Biology Corp. (2000-13) to V.V.D. We thank William Dawson and Gail Wisler for the cDNA clone of CTV and BYSV-infected plant material, respectively, and Valera Peremyslov for assistance with the confocal laser scanning microscopy.
PY - 2003/2/15
Y1 - 2003/2/15
N2 - Using an Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by p21 under these conditions had no apparent effect on accumulation of the small interfering RNAs. In addition, p21 elevated the transient expression level of the GFP mRNA in the absence of dsRNA inducer. Similar activities were detected using homologs of p21 encoded by other members of the genus Closterovirus. Computer analysis indicated that p21-like proteins constitute a novel protein family that is unrelated to other recognized suppressors of RNA silencing. Examination of the subcellular distribution in BYV-infected plants revealed that p21 is partitioned between soluble cytoplasmic form and proteinaceous inclusion bodies at the cell periphery.
AB - Using an Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by p21 under these conditions had no apparent effect on accumulation of the small interfering RNAs. In addition, p21 elevated the transient expression level of the GFP mRNA in the absence of dsRNA inducer. Similar activities were detected using homologs of p21 encoded by other members of the genus Closterovirus. Computer analysis indicated that p21-like proteins constitute a novel protein family that is unrelated to other recognized suppressors of RNA silencing. Examination of the subcellular distribution in BYV-infected plants revealed that p21 is partitioned between soluble cytoplasmic form and proteinaceous inclusion bodies at the cell periphery.
UR - https://www.scopus.com/pages/publications/0037440718
U2 - 10.1016/S0042-6822(02)00051-X
DO - 10.1016/S0042-6822(02)00051-X
M3 - Article
C2 - 12642093
AN - SCOPUS:0037440718
SN - 0042-6822
VL - 306
SP - 203
EP - 209
JO - Virology
JF - Virology
IS - 2
ER -