TY - JOUR
T1 - Suppression of parathyroid hormone secretion by aluminum
AU - Morrissey, J.
AU - Rothstein, M.
AU - Mayor, G.
AU - Slatopolsky, E.
N1 - Funding Information:
A preliminary report of this study was presented at the 14th Annual Meeting, American Society of Nephrology, Washington, D.C., Novem- ber 22—24, 1981. This work was supported by United States Public Health Service NIAMDD Grants AM-09976, AM-07 126, and AM-30 178 and Washington University Biomedical Research Support Grant 51054A. The authors thank Drs. S. Klahr and K. Martin for critical discussions during the course of this work and Mrs. P. Verplancke for assistance in the preparation of this manuscript.
PY - 1983
Y1 - 1983
N2 - The effect of aluminum on parathyroid hormone secretion was examined using collagenase-dispersed bovine parathyroid cells. An increase in the medium aluminum concentration over the range of 0.5 to 2.0 mM, in low calcium medium, progressively inhibited the secretion of radioimmunoassayable hormone. At 2.0 mM aluminum hormone secretion was inhibited by 68% while high medium calcium, without aluminum, maximally inhibited parathyroid hormone secretion only 39%. Individually, 2.0 mM aluminum or 2.0 mM calcium inhibited isoproterenol-stimulated hormone secretion by 43%. Either metal suppressed basal and isoproterenol-stimulated cyclic AMP levels of the parathyroid cells. That the inhibitory effect of aluminum on parathyroid hormone secretion was not due to an irreversible toxic effect was demonstrated by a restoration of normal secretion when cells were returned to 0.5 mM calcium medium without aluminum. The incorporation of [3H]leucine into total cell protein, parathyroid secretory protein, proparathyroid hormone, or parathyroid hormone was not affected by aluminum. The secretion of radiolabeled protein was, however, inhibited by aluminum. These results suggest that aluminum does not affect protein biosynthesis of the parathyroid cell or the conversion of proparathyroid hormone to parathyroid hormone. Aluminum appears to directly affect the secretion of protein from dispersed parathyroid cells.
AB - The effect of aluminum on parathyroid hormone secretion was examined using collagenase-dispersed bovine parathyroid cells. An increase in the medium aluminum concentration over the range of 0.5 to 2.0 mM, in low calcium medium, progressively inhibited the secretion of radioimmunoassayable hormone. At 2.0 mM aluminum hormone secretion was inhibited by 68% while high medium calcium, without aluminum, maximally inhibited parathyroid hormone secretion only 39%. Individually, 2.0 mM aluminum or 2.0 mM calcium inhibited isoproterenol-stimulated hormone secretion by 43%. Either metal suppressed basal and isoproterenol-stimulated cyclic AMP levels of the parathyroid cells. That the inhibitory effect of aluminum on parathyroid hormone secretion was not due to an irreversible toxic effect was demonstrated by a restoration of normal secretion when cells were returned to 0.5 mM calcium medium without aluminum. The incorporation of [3H]leucine into total cell protein, parathyroid secretory protein, proparathyroid hormone, or parathyroid hormone was not affected by aluminum. The secretion of radiolabeled protein was, however, inhibited by aluminum. These results suggest that aluminum does not affect protein biosynthesis of the parathyroid cell or the conversion of proparathyroid hormone to parathyroid hormone. Aluminum appears to directly affect the secretion of protein from dispersed parathyroid cells.
UR - http://www.scopus.com/inward/record.url?scp=0020586923&partnerID=8YFLogxK
U2 - 10.1038/ki.1983.81
DO - 10.1038/ki.1983.81
M3 - Article
C2 - 6308327
AN - SCOPUS:0020586923
SN - 0085-2538
VL - 23
SP - 699
EP - 704
JO - Kidney International
JF - Kidney International
IS - 5
ER -