Supercomputing for the parallelization of whole genome analysis

Megan J. Puckelwartz, Lorenzo L. Pesce, Viswateja Nelakuditi, Lisa Dellefave-Castillo, Jessica R. Golbus, Sharlene M. Day, Thomas P. Cappola, Gerald W. Dorn, Ian T. Foster, Elizabeth M. Mcnally

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Motivation: The declining cost of generating DNA sequence is promoting an increase in whole genome sequencing, especially as applied to the human genome. Whole genome analysis requires the alignment and comparison of raw sequence data, and results in a computational bottleneck because of limited ability to analyze multiple genomes simultaneously. Results: We now adapted a Cray XE6 supercomputer to achieve the parallelization required for concurrent multiple genome analysis. This approach not only markedly speeds computational time but also results in increased usable sequence per genome. Relying on publically available software, the Cray XE6 has the capacity to align and call variants on 240 whole genomes in ∼50 h. Multisample variant calling is also accelerated.

Original languageEnglish
Pages (from-to)1508-1513
Number of pages6
JournalBioinformatics
Volume30
Issue number11
DOIs
StatePublished - Jun 1 2014

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