Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

Zhen Liu; Quynh P.H. Nguyen; Rashmi Nanjundappa; Nathalie Delgehyr; Alexandre Megherbi; Regan Doherty; James Thompson; Claire Jackson; Alexandra Albulescu; Yew M. Heng; Jane S. Lucas; Sharon D. Dell; Alice Meunier; Kirk Czymmek; Moe R. Mahjoub; Vito Mennella

doi:10.1016/j.devcel.2020.09.016

Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

Zhen Liu
, Quynh P.H. Nguyen
, Rashmi Nanjundappa
, Nathalie Delgehyr
, Alexandre Megherbi
, Regan Doherty
, James Thompson
, Claire Jackson
, Alexandra Albulescu
, Yew M. Heng
, Jane S. Lucas
, Sharon D. Dell
, Alice Meunier
, Kirk Czymmek
, Moe R. Mahjoub
, Vito Mennella

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.

Original language	English
Pages (from-to)	224-236.e6
Journal	Developmental cell
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/j.devcel.2020.09.016
State	Published - Oct 26 2020

Keywords

FIB-SEM
airway
appendages
basal bodies
basal foot
centrosome
cilia
electron microscopy
primary ciliary dyskinesia
quantitative imaging
super-resolution imaging

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10.1016/j.devcel.2020.09.016

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Liu, Z., Nguyen, Q. P. H., Nanjundappa, R., Delgehyr, N., Megherbi, A., Doherty, R., Thompson, J., Jackson, C., Albulescu, A., Heng, Y. M., Lucas, J. S., Dell, S. D., Meunier, A., Czymmek, K., Mahjoub, M. R., & Mennella, V. (2020). Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells. Developmental cell, 55(2), 224-236.e6. https://doi.org/10.1016/j.devcel.2020.09.016

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title = "Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells",

abstract = "Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.",

keywords = "FIB-SEM, airway, appendages, basal bodies, basal foot, centrosome, cilia, electron microscopy, primary ciliary dyskinesia, quantitative imaging, super-resolution imaging",

author = "Zhen Liu and Nguyen, \{Quynh P.H.\} and Rashmi Nanjundappa and Nathalie Delgehyr and Alexandre Megherbi and Regan Doherty and James Thompson and Claire Jackson and Alexandra Albulescu and Heng, \{Yew M.\} and Lucas, \{Jane S.\} and Dell, \{Sharon D.\} and Alice Meunier and Kirk Czymmek and Mahjoub, \{Moe R.\} and Vito Mennella",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = oct,

day = "26",

doi = "10.1016/j.devcel.2020.09.016",

language = "English",

volume = "55",

pages = "224--236.e6",

journal = "Developmental cell",

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Liu, Z, Nguyen, QPH, Nanjundappa, R, Delgehyr, N, Megherbi, A, Doherty, R, Thompson, J, Jackson, C, Albulescu, A, Heng, YM, Lucas, JS, Dell, SD, Meunier, A, Czymmek, K, Mahjoub, MR & Mennella, V 2020, 'Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells', Developmental cell, vol. 55, no. 2, pp. 224-236.e6. https://doi.org/10.1016/j.devcel.2020.09.016

TY - JOUR

T1 - Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

AU - Liu, Zhen

AU - Nguyen, Quynh P.H.

AU - Nanjundappa, Rashmi

AU - Delgehyr, Nathalie

AU - Megherbi, Alexandre

AU - Doherty, Regan

AU - Thompson, James

AU - Jackson, Claire

AU - Albulescu, Alexandra

AU - Heng, Yew M.

AU - Lucas, Jane S.

AU - Dell, Sharon D.

AU - Meunier, Alice

AU - Czymmek, Kirk

AU - Mahjoub, Moe R.

AU - Mennella, Vito

PY - 2020/10/26

Y1 - 2020/10/26

N2 - Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.

AB - Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.

KW - FIB-SEM

KW - airway

KW - appendages

KW - basal bodies

KW - basal foot

KW - centrosome

KW - cilia

KW - electron microscopy

KW - primary ciliary dyskinesia

KW - quantitative imaging

KW - super-resolution imaging

UR - https://www.scopus.com/pages/publications/85093114738

U2 - 10.1016/j.devcel.2020.09.016

DO - 10.1016/j.devcel.2020.09.016

M3 - Article

C2 - 33038333

AN - SCOPUS:85093114738

SN - 1534-5807

VL - 55

SP - 224-236.e6

JO - Developmental cell

JF - Developmental cell

IS - 2

ER -

Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

Abstract

Keywords

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