@article{0c13eb604a2a487aa293f92acf72c629,
title = "Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells",
abstract = "Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.",
keywords = "FIB-SEM, airway, appendages, basal bodies, basal foot, centrosome, cilia, electron microscopy, primary ciliary dyskinesia, quantitative imaging, super-resolution imaging",
author = "Zhen Liu and Nguyen, {Quynh P.H.} and Rashmi Nanjundappa and Nathalie Delgehyr and Alexandre Megherbi and Regan Doherty and James Thompson and Claire Jackson and Alexandra Albulescu and Heng, {Yew M.} and Lucas, {Jane S.} and Dell, {Sharon D.} and Alice Meunier and Kirk Czymmek and Mahjoub, {Moe R.} and Vito Mennella",
note = "Funding Information: This project is funded by CIHR program grant # 391917 to V.M. and S.D. and National Heart Lung and Blood Institute ( R01-HL128370 ) and the Children{\textquoteright}s Discovery Institute of Washington University and St. Louis Children{\textquoteright}s Hospital ( CDI-CORE-2019-813 ) to M.R.M. Z.L. was supported by the SickKids Restracomp Fellowship. The authors acknowledge PCD patients and volunteers for providing nasal cells for this study and Julie Avolio for help with nasal cell scraping. Jia Zhou, Cindy Fang, and Jasmine Kang assisted in data analysis. Douglas Holmyard (EM facility, The Hospital for Sick Children) prepared TEM and FIB-SEM samples and helped set up EM imaging. McGill EM facility contributed to FIB-SEM acquisition. We thank Profs Sudipto Roi{\textquoteright}s lab, Jeremy Reiter{\textquoteright}s lab, and Nick Berbari{\textquoteright}s lab for experimental help. We thank Profs Bornens, Pelletier, Cheeseman, Kyung Lee, Elsasser, and Avidor-Reiss laboratories for sharing antibodies and plasmids. Funding Information: This project is funded by CIHR program grant # 391917 to V.M. and S.D. and National Heart Lung and Blood Institute (R01-HL128370) and the Children's Discovery Institute of Washington University and St. Louis Children's Hospital (CDI-CORE-2019-813) to M.R.M. Z.L. was supported by the SickKids Restracomp Fellowship. The authors acknowledge PCD patients and volunteers for providing nasal cells for this study and Julie Avolio for help with nasal cell scraping. Jia Zhou, Cindy Fang, and Jasmine Kang assisted in data analysis. Douglas Holmyard (EM facility, The Hospital for Sick Children) prepared TEM and FIB-SEM samples and helped set up EM imaging. McGill EM facility contributed to FIB-SEM acquisition. We thank Profs Sudipto Roi's lab, Jeremy Reiter's lab, and Nick Berbari's lab for experimental help. We thank Profs Bornens, Pelletier, Cheeseman, Kyung Lee, Elsasser, and Avidor-Reiss laboratories for sharing antibodies and plasmids. Z.L. and Q.P.H.N. conducted experiments, collected and analyzed the data, wrote methods, and edited the manuscript. A.A. helped with the airway multiciliated cells culturing system; R.N. performed the mouse tracheal epithelia cell experiments; J.T. performed ex vivo trachea labeling experiments; C.J. helped with trachea labeling and edited the manuscript; R.D. and Y.H. performed TEM on CCNO PCD patients; M.M. and V.M. conceived and designed the MTEC-centrinone experiments; N.D. M.A. and A.M. conceived and performed the ependymal cells pulse-chase experiments; S.D. and J.S.L. provided PCD patients cells; K.C. helped with sample preparation for FIB-SEM data collection and acquired data; V.M. conceived the project, designed experiments, analyzed data, and wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = oct,
day = "26",
doi = "10.1016/j.devcel.2020.09.016",
language = "English",
volume = "55",
pages = "224--236.e6",
journal = "Developmental cell",
issn = "1534-5807",
number = "2",
}