Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

Zhen Liu; Quynh P.H. Nguyen; Rashmi Nanjundappa; Nathalie Delgehyr; Alexandre Megherbi; Regan Doherty; James Thompson; Claire Jackson; Alexandra Albulescu; Yew M. Heng; Jane S. Lucas; Sharon D. Dell; Alice Meunier; Kirk Czymmek; Moe R. Mahjoub; Vito Mennella

doi:10.1016/j.devcel.2020.09.016

Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

Zhen Liu, Quynh P.H. Nguyen, Rashmi Nanjundappa, Nathalie Delgehyr, Alexandre Megherbi, Regan Doherty, James Thompson, Claire Jackson, Alexandra Albulescu, Yew M. Heng, Jane S. Lucas, Sharon D. Dell, Alice Meunier, Kirk Czymmek, Moe R. Mahjoub, Vito Mennella

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23 Scopus citations

Abstract

Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.

Original language	English
Pages (from-to)	224-236.e6
Journal	Developmental cell
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/j.devcel.2020.09.016
State	Published - Oct 26 2020

Keywords

FIB-SEM
airway
appendages
basal bodies
basal foot
centrosome
cilia
electron microscopy
primary ciliary dyskinesia
quantitative imaging
super-resolution imaging

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10.1016/j.devcel.2020.09.016

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Liu, Z., Nguyen, Q. P. H., Nanjundappa, R., Delgehyr, N., Megherbi, A., Doherty, R., Thompson, J., Jackson, C., Albulescu, A., Heng, Y. M., Lucas, J. S., Dell, S. D., Meunier, A., Czymmek, K., Mahjoub, M. R., & Mennella, V. (2020). Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells. Developmental cell, 55(2), 224-236.e6. https://doi.org/10.1016/j.devcel.2020.09.016

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title = "Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells",

abstract = "Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.",

keywords = "FIB-SEM, airway, appendages, basal bodies, basal foot, centrosome, cilia, electron microscopy, primary ciliary dyskinesia, quantitative imaging, super-resolution imaging",

author = "Zhen Liu and Nguyen, {Quynh P.H.} and Rashmi Nanjundappa and Nathalie Delgehyr and Alexandre Megherbi and Regan Doherty and James Thompson and Claire Jackson and Alexandra Albulescu and Heng, {Yew M.} and Lucas, {Jane S.} and Dell, {Sharon D.} and Alice Meunier and Kirk Czymmek and Mahjoub, {Moe R.} and Vito Mennella",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = oct,

day = "26",

doi = "10.1016/j.devcel.2020.09.016",

language = "English",

volume = "55",

pages = "224--236.e6",

journal = "Developmental cell",

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Liu, Z, Nguyen, QPH, Nanjundappa, R, Delgehyr, N, Megherbi, A, Doherty, R, Thompson, J, Jackson, C, Albulescu, A, Heng, YM, Lucas, JS, Dell, SD, Meunier, A, Czymmek, K, Mahjoub, MR & Mennella, V 2020, 'Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells', Developmental cell, vol. 55, no. 2, pp. 224-236.e6. https://doi.org/10.1016/j.devcel.2020.09.016

TY - JOUR

T1 - Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

AU - Liu, Zhen

AU - Nguyen, Quynh P.H.

AU - Nanjundappa, Rashmi

AU - Delgehyr, Nathalie

AU - Megherbi, Alexandre

AU - Doherty, Regan

AU - Thompson, James

AU - Jackson, Claire

AU - Albulescu, Alexandra

AU - Heng, Yew M.

AU - Lucas, Jane S.

AU - Dell, Sharon D.

AU - Meunier, Alice

AU - Czymmek, Kirk

AU - Mahjoub, Moe R.

AU - Mennella, Vito

PY - 2020/10/26

Y1 - 2020/10/26

N2 - Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.

AB - Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin, and cellular position, and it participates in basal body alignment.

KW - FIB-SEM

KW - airway

KW - appendages

KW - basal bodies

KW - basal foot

KW - centrosome

KW - cilia

KW - electron microscopy

KW - primary ciliary dyskinesia

KW - quantitative imaging

KW - super-resolution imaging

UR - http://www.scopus.com/inward/record.url?scp=85093114738&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2020.09.016

DO - 10.1016/j.devcel.2020.09.016

M3 - Article

C2 - 33038333

AN - SCOPUS:85093114738

SN - 1534-5807

VL - 55

SP - 224-236.e6

JO - Developmental cell

JF - Developmental cell

IS - 2

ER -

Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells

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Keywords

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