Super cool X-1000 and Super cool Z-1000, two ice blockers, and their effect on vitrification/warming of mouse embryos

H. Badrzadeh, S. Najmabadi, R. Paymani, T. Macaso, Z. Azadbadi, A. Ahmady

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Objective: To evaluate the survival and blastocyst formation rates of mouse embryos after vitrification/thaw process with different ice blocker media. We used X-1000 and Z-1000 separately and mixed using V-Kim, a closed vitrification system. Study design: Mouse embryos were vitrified using ethylene glycol based medium supplemented with Super cool X-1000 and/or Super cool Z-1000. Results: Survival rates for the control, Super cool X-1000, Super cool Z-1000, and Super cool X-1000/Z-1000 groups were 74%, 72%, 68%, and 85% respectively, with no significant difference among experimental and control groups; however, a significantly higher survival rate was noticed in the Super cool X-1000/Z-1000 group when compared with the Super cool Z-1000 group. Blastocyst formation rates for the control, Super cool X-1000, Super cool Z-1000, and Super cool X-1000/Z-1000 groups were 71%, 66%, 65%, and 72% respectively. There was no significant difference in this rate among control and experimental groups. Conclusions: In a closed vitrification system, addition of ice blocker Super cool X-1000 to the vitrification solution containing Super cool Z-1000 may improve the embryo survival rate. We recommend combined ice blocker usage to optimize the vitrification outcome.

Original languageEnglish
Pages (from-to)70-71
Number of pages2
JournalEuropean Journal of Obstetrics and Gynecology and Reproductive Biology
Volume151
Issue number1
DOIs
StatePublished - Jul 2010

Keywords

  • Closed system
  • Embryo
  • Mouse
  • Super cool X-1000
  • Super cool Z-1000
  • Survival rate
  • Vitrification

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