Sugar additives for MALDI matrices improve signal allowing the smallest nucleotide change (A:T) in a DNA sequence to be resolved

Keyphrases

• DNA Sequencing 100%
• Matrix-assisted Laser Desorption Ionization (MALDI) 100%
• Laser Fluence 100%
• Sample Preparation 66%
• Crystallization 66%
• DNA Molecule 66%
• Mass Resolution 66%
• Laser Energy 66%
• Single nucleotide Polymorphism 33%
• Fucose 33%
• Substrate Surface 33%
• Environmental Conditions 33%
• Mass Difference 33%
• Primer Extension 33%
• Energy Increase 33%
• Mass Spectra 33%
• Humidity 33%
• Automatic Measurement 33%
• Single nucleotide 33%
• Fructose 33%
• Impurities 33%
• Co-crystallization 33%
• Excess Energy 33%
• Time-of-flight Mass Spectrometry 33%
• Surface Condition 33%
• Effective Matrix 33%
• Desorption Ionization 33%
• Fluence Threshold 33%
• Matrix-assisted Laser Desorption Ionization Mass Spectrometry 33%
• Solvent Content 33%
• 3-Hydroxypicolinic Acid 33%
• Automatic Data Acquisition 33%
• Metastable Decay 33%

Chemistry

• Matrix-Assisted Laser Desorption-Ionization 100%
• DNA Sequence 100%
• DNA 100%
• Co Crystallization 25%
• Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry 25%
• Time of Flight Mass Spectroscopy 25%
• 3-Hydroxypicolinic Acid 25%
• Desorption 25%
• Fucose 25%
• Fructose 25%