Subtraction by addition: Domesticated transposases in programmed DNA elimination

Jason A. Motl; Douglas L. Chalker

doi:10.1101/gad.1864609

Subtraction by addition: Domesticated transposases in programmed DNA elimination

Jason A. Motl, Douglas L. Chalker

Research output: Contribution to journal › Review article › peer-review

4 Scopus citations

Abstract

The ciliate Paramecium tetraurelia must eliminate ∼60,000 short sequences from its genome to generate uninterrupted coding sequences in its somatic macronucleus. In this issue of Genes & Development, Baudry and colleagues (pp. 2478-2483) identify the protein that excises these noncoding sequences: a domesticated piggyBac transposase that has been adapted to remove what are likely the remnants of transposon insertions. This new study reveals how addition of a transposase to small RNA-directed silencing machinery can guide major genome reorganization.

Original language	English
Pages (from-to)	2455-2460
Number of pages	6
Journal	Genes and Development
Volume	23
Issue number	21
DOIs	https://doi.org/10.1101/gad.1864609
State	Published - Nov 1 2009

Keywords

Chromosome fragmentation
IES excision
Oligohymenophorean ciliates
Transposase domestication

Access to Document

10.1101/gad.1864609

Cite this

@article{e11ff536a32e48008fc033e9636170b5,

title = "Subtraction by addition: Domesticated transposases in programmed DNA elimination",

abstract = "The ciliate Paramecium tetraurelia must eliminate ∼60,000 short sequences from its genome to generate uninterrupted coding sequences in its somatic macronucleus. In this issue of Genes & Development, Baudry and colleagues (pp. 2478-2483) identify the protein that excises these noncoding sequences: a domesticated piggyBac transposase that has been adapted to remove what are likely the remnants of transposon insertions. This new study reveals how addition of a transposase to small RNA-directed silencing machinery can guide major genome reorganization.",

keywords = "Chromosome fragmentation, IES excision, Oligohymenophorean ciliates, Transposase domestication",

author = "Motl, {Jason A.} and Chalker, {Douglas L.}",

year = "2009",

month = nov,

day = "1",

doi = "10.1101/gad.1864609",

language = "English",

volume = "23",

pages = "2455--2460",

journal = "Genes and Development",

issn = "0890-9369",

number = "21",

}

TY - JOUR

T1 - Subtraction by addition

T2 - Domesticated transposases in programmed DNA elimination

AU - Motl, Jason A.

AU - Chalker, Douglas L.

PY - 2009/11/1

Y1 - 2009/11/1

N2 - The ciliate Paramecium tetraurelia must eliminate ∼60,000 short sequences from its genome to generate uninterrupted coding sequences in its somatic macronucleus. In this issue of Genes & Development, Baudry and colleagues (pp. 2478-2483) identify the protein that excises these noncoding sequences: a domesticated piggyBac transposase that has been adapted to remove what are likely the remnants of transposon insertions. This new study reveals how addition of a transposase to small RNA-directed silencing machinery can guide major genome reorganization.

AB - The ciliate Paramecium tetraurelia must eliminate ∼60,000 short sequences from its genome to generate uninterrupted coding sequences in its somatic macronucleus. In this issue of Genes & Development, Baudry and colleagues (pp. 2478-2483) identify the protein that excises these noncoding sequences: a domesticated piggyBac transposase that has been adapted to remove what are likely the remnants of transposon insertions. This new study reveals how addition of a transposase to small RNA-directed silencing machinery can guide major genome reorganization.

KW - Chromosome fragmentation

KW - IES excision

KW - Oligohymenophorean ciliates

KW - Transposase domestication

UR - http://www.scopus.com/inward/record.url?scp=70350654481&partnerID=8YFLogxK

U2 - 10.1101/gad.1864609

DO - 10.1101/gad.1864609

M3 - Review article

C2 - 19884252

AN - SCOPUS:70350654481

SN - 0890-9369

VL - 23

SP - 2455

EP - 2460

JO - Genes and Development

JF - Genes and Development

IS - 21

ER -

Subtraction by addition: Domesticated transposases in programmed DNA elimination

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this