Study of the role of schiff base formation in the aromatization of 3-[18O]testosterone and 3,17-DI-[18O]androstenedione by human placental aromatase

Denise D. Beusen; Douglas F. Covey

doi:10.1016/0022-4731(84)90408-4

Study of the role of schiff base formation in the aromatization of 3-[¹⁸O]testosterone and 3,17-DI-[¹⁸O]androstenedione by human placental aromatase

Denise D. Beusen
, Douglas F. Covey

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Testosterone, prepared with ¹⁸O at the 3 position, and androstenedione, prepared with ¹⁸O at both the 3 and 17 positions, were incubated with human placental microsomes. The resulting estrogen metabolites (estradiol and estrone, respectively) as well as unconverted starting material were isolated and analyzed for their ¹⁸O content by gas chromatography-mass spectrometry. In each case, > 90% of the ¹⁸O present in the original substrate was retained in the products. This result argues against a role for Schiff base formation in the aromatase reaction.

Original language	English
Pages (from-to)	931-934
Number of pages	4
Journal	Journal of Steroid Biochemistry
Volume	20
Issue number	4 PART 1
DOIs	https://doi.org/10.1016/0022-4731(84)90408-4
State	Published - Apr 1984

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title = "Study of the role of schiff base formation in the aromatization of 3-[18O]testosterone and 3,17-DI-[18O]androstenedione by human placental aromatase",

abstract = "Testosterone, prepared with 18O at the 3 position, and androstenedione, prepared with 18O at both the 3 and 17 positions, were incubated with human placental microsomes. The resulting estrogen metabolites (estradiol and estrone, respectively) as well as unconverted starting material were isolated and analyzed for their 18O content by gas chromatography-mass spectrometry. In each case, > 90\% of the 18O present in the original substrate was retained in the products. This result argues against a role for Schiff base formation in the aromatase reaction.",

author = "Beusen, \{Denise D.\} and Covey, \{Douglas F.\}",

note = "Funding Information: Acknowledgements-This investigation was supported in part by NIH grants CA23582 and RR00954 (Division of Research Res\&rces). DFC is the recipient of a National Institutes of Health Research Career Development Award CA00829. DDB is supported by National Institutes of Health training grant 5T32 GM07067.",

year = "1984",

month = apr,

doi = "10.1016/0022-4731(84)90408-4",

language = "English",

volume = "20",

pages = "931--934",

journal = "Journal of Steroid Biochemistry",

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T1 - Study of the role of schiff base formation in the aromatization of 3-[18O]testosterone and 3,17-DI-[18O]androstenedione by human placental aromatase

AU - Beusen, Denise D.

AU - Covey, Douglas F.

N1 - Funding Information: Acknowledgements-This investigation was supported in part by NIH grants CA23582 and RR00954 (Division of Research Res&rces). DFC is the recipient of a National Institutes of Health Research Career Development Award CA00829. DDB is supported by National Institutes of Health training grant 5T32 GM07067.

PY - 1984/4

Y1 - 1984/4

N2 - Testosterone, prepared with 18O at the 3 position, and androstenedione, prepared with 18O at both the 3 and 17 positions, were incubated with human placental microsomes. The resulting estrogen metabolites (estradiol and estrone, respectively) as well as unconverted starting material were isolated and analyzed for their 18O content by gas chromatography-mass spectrometry. In each case, > 90% of the 18O present in the original substrate was retained in the products. This result argues against a role for Schiff base formation in the aromatase reaction.

AB - Testosterone, prepared with 18O at the 3 position, and androstenedione, prepared with 18O at both the 3 and 17 positions, were incubated with human placental microsomes. The resulting estrogen metabolites (estradiol and estrone, respectively) as well as unconverted starting material were isolated and analyzed for their 18O content by gas chromatography-mass spectrometry. In each case, > 90% of the 18O present in the original substrate was retained in the products. This result argues against a role for Schiff base formation in the aromatase reaction.

UR - https://www.scopus.com/pages/publications/0021413546

U2 - 10.1016/0022-4731(84)90408-4

DO - 10.1016/0022-4731(84)90408-4

M3 - Article

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AN - SCOPUS:0021413546

SN - 0022-4731

VL - 20

SP - 931

EP - 934

JO - Journal of Steroid Biochemistry

JF - Journal of Steroid Biochemistry

IS - 4 PART 1

ER -

Study of the role of schiff base formation in the aromatization of 3-[¹⁸O]testosterone and 3,17-DI-[¹⁸O]androstenedione by human placental aromatase

Abstract

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