TY - JOUR
T1 - Study of the role of schiff base formation in the aromatization of 3-[18O]testosterone and 3,17-DI-[18O]androstenedione by human placental aromatase
AU - Beusen, Denise D.
AU - Covey, Douglas F.
N1 - Funding Information:
Acknowledgements-This investigation was supported in part by NIH grants CA23582 and RR00954 (Division of Research Res&rces). DFC is the recipient of a National Institutes of Health Research Career Development Award CA00829. DDB is supported by National Institutes of Health training grant 5T32 GM07067.
PY - 1984/4
Y1 - 1984/4
N2 - Testosterone, prepared with 18O at the 3 position, and androstenedione, prepared with 18O at both the 3 and 17 positions, were incubated with human placental microsomes. The resulting estrogen metabolites (estradiol and estrone, respectively) as well as unconverted starting material were isolated and analyzed for their 18O content by gas chromatography-mass spectrometry. In each case, > 90% of the 18O present in the original substrate was retained in the products. This result argues against a role for Schiff base formation in the aromatase reaction.
AB - Testosterone, prepared with 18O at the 3 position, and androstenedione, prepared with 18O at both the 3 and 17 positions, were incubated with human placental microsomes. The resulting estrogen metabolites (estradiol and estrone, respectively) as well as unconverted starting material were isolated and analyzed for their 18O content by gas chromatography-mass spectrometry. In each case, > 90% of the 18O present in the original substrate was retained in the products. This result argues against a role for Schiff base formation in the aromatase reaction.
UR - http://www.scopus.com/inward/record.url?scp=0021413546&partnerID=8YFLogxK
U2 - 10.1016/0022-4731(84)90408-4
DO - 10.1016/0022-4731(84)90408-4
M3 - Article
C2 - 6708562
AN - SCOPUS:0021413546
SN - 0022-4731
VL - 20
SP - 931
EP - 934
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 4 PART 1
ER -