TY - JOUR
T1 - Studies on the biosynthesis of dolichyl phosphate
T2 - Evidence for the in vitro formation of 2,3-dehydrodolichyl phosphate
AU - Grange, Dorothy K.
AU - Lee Adair, W.
PY - 1977/12/7
Y1 - 1977/12/7
N2 - A particulate enzyme preparation from hen oviduct is shown to carry out the biosynthesis of a long chain polyprenyl phosphate from isopentenyl pyrophosphate and farnesyl pyrophosphate. The compound has the physical and chemical properties of 2,3-dehydrodolichyl phosphate. The enzyme system is inhibited by EDTA and stimulated by Triton X-100 and dithiothreitol. If the product of the reaction is 2,3-dehydrodolichyl phosphate, it may be derived from 2,3-dehydrodolichyl pyrophosphate, a likely intermediate in the biosynthesis of dolichyl phosphate.
AB - A particulate enzyme preparation from hen oviduct is shown to carry out the biosynthesis of a long chain polyprenyl phosphate from isopentenyl pyrophosphate and farnesyl pyrophosphate. The compound has the physical and chemical properties of 2,3-dehydrodolichyl phosphate. The enzyme system is inhibited by EDTA and stimulated by Triton X-100 and dithiothreitol. If the product of the reaction is 2,3-dehydrodolichyl phosphate, it may be derived from 2,3-dehydrodolichyl pyrophosphate, a likely intermediate in the biosynthesis of dolichyl phosphate.
UR - http://www.scopus.com/inward/record.url?scp=0017651223&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(77)91173-1
DO - 10.1016/0006-291X(77)91173-1
M3 - Article
C2 - 597303
AN - SCOPUS:0017651223
SN - 0006-291X
VL - 79
SP - 734
EP - 740
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -