Studies on Antibodies Directed toward Single Antigenic Sites on Globular Proteins

Robert W. Noble, Morris Reichlin, Robert D. Schreiber

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We have isolated an antibody population which reacts with hemoglobin S but not with hemoglobin A. These proteins differ by a single amino acid substitution. We have isolated an antibody population which reacts with human cytochrome c but not with monkey cytochrome c. These proteins also differ by a single amino acid residue. These antibody populations react with their specific antigens with a stoichiometry of one antibody combining site per asymetrical antigenic unit. A second, different antibody population which also reacts with human cytochrome c with a stoichiometry of unity has been isolated by virtue of its excess production. The kinetics of reaction of these site specific antibody populations indicates considerable homogeneity. This study represents the first isolation of antibodies specific to single chemical modifications of globular proteins and the first demonstration of a one to one binding stoichiometry between such an antigen and an antibody population.

Original languageEnglish
Pages (from-to)3326-3332
Number of pages7
JournalBiochemistry
Volume11
Issue number18
DOIs
StatePublished - Aug 1 1972

Fingerprint

Dive into the research topics of 'Studies on Antibodies Directed toward Single Antigenic Sites on Globular Proteins'. Together they form a unique fingerprint.

Cite this