Studies of the Enzyme Fumarase. II.1 Isolation and Physical Properties of Crystalline Enzyme

Car Frieden, Robert M. Bock, Robert A. Alberty

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A method is described for the isolation and crystallization of fumarase from pig heart muscle which differs from methods which have previously been reported. Based on the muscle extract, a 2600-fold increase in specific activity and a recovery of 20% are obtained. This protein yields a single boundary in the electrophoresis apparatus and in the velocity ultracentrifuge. The ultraviolet absorption is characteristic of a protein, and it has not been possible to detect an enzyme-substrate complex spectrophotometrically.

Original languageEnglish
Pages (from-to)2482-2484
Number of pages3
JournalJournal of the American Chemical Society
Volume76
Issue number9
DOIs
StatePublished - May 1954
Externally publishedYes

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