TY - JOUR
T1 - Studies of the biosynthesis of the cation-dependent mannose 6-phosphate receptor in murine cell lines
AU - Gasa, Shinsei
AU - Kornfeld, Stuart
N1 - Funding Information:
i This investigation was supported CA08759 from the U.S. Public Health by a Monsanto Company/Washington Biomedical Research Grant. a Present address: Cancer Institute, versity School of Medicine, Sapporo, a To whom correspondence should 4 Abbreviations used: Man-6-P dependent mannose 6-phosphate sin inhibitor unit; Con A, concanavalin minimal essential medium; endo griseus endo+N-acetylglucosaminidase Flaw0 bacterium endo-P-N-acetylglucosaminidase SDS, sodium dodecyl sulfate; Hepes, ethyl)-1-piperazineethanesulfonic acid.
PY - 1987/8/15
Y1 - 1987/8/15
N2 - We have studied the biosynthesis of the cation-dependent mannose 6-phosphate receptor in murine BW5147 lymphoma cells and MOPC 315 plasmacytoma cells. The cells were labeled with [35S]methionine or [2-3H]mannose and the receptor immunoprecipitated with an anti-receptor antiserum. The receptor was first detected as a glycoprotein with an apparent molecular mass of 40 kDa. This intermediate was rapidly processed to a mature form which was stable during 22 h of chase. In these cells, the mature receptor has an apparent molecular mass of 43 kDa. The 3-kDa increase occurs as a result of processing of Asn-linked high-mannose oligosaccharides to complex-type units.
AB - We have studied the biosynthesis of the cation-dependent mannose 6-phosphate receptor in murine BW5147 lymphoma cells and MOPC 315 plasmacytoma cells. The cells were labeled with [35S]methionine or [2-3H]mannose and the receptor immunoprecipitated with an anti-receptor antiserum. The receptor was first detected as a glycoprotein with an apparent molecular mass of 40 kDa. This intermediate was rapidly processed to a mature form which was stable during 22 h of chase. In these cells, the mature receptor has an apparent molecular mass of 43 kDa. The 3-kDa increase occurs as a result of processing of Asn-linked high-mannose oligosaccharides to complex-type units.
UR - http://www.scopus.com/inward/record.url?scp=0023655889&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(87)90555-8
DO - 10.1016/0003-9861(87)90555-8
M3 - Article
C2 - 2957959
AN - SCOPUS:0023655889
SN - 0003-9861
VL - 257
SP - 170
EP - 176
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -