Structure of the complex oligosaccharides of fetuin

J. U. Baenziger, D. Fiete

Research output: Contribution to journalArticle

170 Scopus citations

Abstract

The complete structure of the complex oligosaccharides of fetuin has been established. The three fractions of complex oligosaccharide which were isolated by ion exchange chromatography following pronase digestion (F-I, F-II, and F-III) had identical molar ratios of sialic acid (Sia), galactose, mannose, and N-acetylglucosamine of 3:3:3:5. A combination of methylation analyses, Smith periodate degradations, and endoglycosidase and exoglycosidase digestions were utilized to establish the structure. Features of this structure not previously established include the presence of 2 residues of α2,3- and 1 residue of α2,6-linked sialic acid and their location relative to the mannose branch points. Also unusual is the presence of an α-linked branch mannose with substituents at positions 2 and 4 which is in turn linked to position 6 of the β-linked, branch mannose. These features result in unexpected resistance to specific exoglycosidases.

Original languageEnglish
Pages (from-to)789-795
Number of pages7
JournalJournal of Biological Chemistry
Volume254
Issue number3
StatePublished - Jan 1 1979

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