Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme

Tao Wang; Xiangbin Zhang; Poonam Bheda; Javier R. Revollo; Shin Ichiro Imai; Cynthia Wolberger

doi:10.1038/nsmb1114

Structure of Nampt/PBEF/visfatin, a mammalian NAD⁺ biosynthetic enzyme

Tao Wang
, Xiangbin Zhang
, Poonam Bheda
, Javier R. Revollo
, Shin Ichiro Imai
, Cynthia Wolberger

Research output: Contribution to journal › Article › peer-review

264 Scopus citations

Abstract

Nicotinamide phosphoribosyltransferase (Nampt) synthesizes nicotinamide mononucleotide (NMN) from nicotinamide in a mammalian NAD⁺ biosynthetic pathway and is required for SirT1 activity in vivo. Nampt has also been presumed to be a cytokine (PBEF) or a hormone (visfatin). The crystal structure of Nampt in the presence and absence of NMN shows that Nampt is a dimeric type II phosphoribosyltransferase and provides insights into the enzymatic mechanism.

Original language	English
Pages (from-to)	661-662
Number of pages	2
Journal	Nature Structural and Molecular Biology
Volume	13
Issue number	7
DOIs	https://doi.org/10.1038/nsmb1114
State	Published - Jul 2006

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title = "Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme",

abstract = "Nicotinamide phosphoribosyltransferase (Nampt) synthesizes nicotinamide mononucleotide (NMN) from nicotinamide in a mammalian NAD+ biosynthetic pathway and is required for SirT1 activity in vivo. Nampt has also been presumed to be a cytokine (PBEF) or a hormone (visfatin). The crystal structure of Nampt in the presence and absence of NMN shows that Nampt is a dimeric type II phosphoribosyltransferase and provides insights into the enzymatic mechanism.",

author = "Tao Wang and Xiangbin Zhang and Poonam Bheda and Revollo, \{Javier R.\} and Imai, \{Shin Ichiro\} and Cynthia Wolberger",

note = "Funding Information: We thank the staff at GM/CA-CAT at the Advanced Photon Source, which is funded in part by the US National Cancer Institute (Y1-CO-1020) and the US National Institute of General Medical Sciences (Y1-GM-1104). J.R.R. is a fellow supported by the Lucille P. Markey Special Emphasis Pathway in Human Pathology. S.I. is an Ellison Medical Foundation Scholar in Aging.",

year = "2006",

month = jul,

doi = "10.1038/nsmb1114",

language = "English",

volume = "13",

pages = "661--662",

journal = "Nature Structural and Molecular Biology",

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T1 - Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme

AU - Wang, Tao

AU - Zhang, Xiangbin

AU - Bheda, Poonam

AU - Revollo, Javier R.

AU - Imai, Shin Ichiro

AU - Wolberger, Cynthia

N1 - Funding Information: We thank the staff at GM/CA-CAT at the Advanced Photon Source, which is funded in part by the US National Cancer Institute (Y1-CO-1020) and the US National Institute of General Medical Sciences (Y1-GM-1104). J.R.R. is a fellow supported by the Lucille P. Markey Special Emphasis Pathway in Human Pathology. S.I. is an Ellison Medical Foundation Scholar in Aging.

PY - 2006/7

Y1 - 2006/7

N2 - Nicotinamide phosphoribosyltransferase (Nampt) synthesizes nicotinamide mononucleotide (NMN) from nicotinamide in a mammalian NAD+ biosynthetic pathway and is required for SirT1 activity in vivo. Nampt has also been presumed to be a cytokine (PBEF) or a hormone (visfatin). The crystal structure of Nampt in the presence and absence of NMN shows that Nampt is a dimeric type II phosphoribosyltransferase and provides insights into the enzymatic mechanism.

AB - Nicotinamide phosphoribosyltransferase (Nampt) synthesizes nicotinamide mononucleotide (NMN) from nicotinamide in a mammalian NAD+ biosynthetic pathway and is required for SirT1 activity in vivo. Nampt has also been presumed to be a cytokine (PBEF) or a hormone (visfatin). The crystal structure of Nampt in the presence and absence of NMN shows that Nampt is a dimeric type II phosphoribosyltransferase and provides insights into the enzymatic mechanism.

UR - https://www.scopus.com/pages/publications/33745862384

U2 - 10.1038/nsmb1114

DO - 10.1038/nsmb1114

M3 - Article

C2 - 16783373

AN - SCOPUS:33745862384

SN - 1545-9993

VL - 13

SP - 661

EP - 662

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

IS - 7

ER -

Structure of Nampt/PBEF/visfatin, a mammalian NAD⁺ biosynthetic enzyme

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