TY - JOUR
T1 - Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD
AU - Thomas, Leonard M.
AU - Harper, Angelica R.
AU - Miner, Whitney A.
AU - Ajufo, Helen O.
AU - Branscum, Katie M.
AU - Kao, Lydia
AU - Sims, Paul A.
PY - 2013/7
Y1 - 2013/7
N2 - The crystal structure of AdhP, a recombinantly expressed alcohol dehydrogenase from Escherichia coli K-12 (substrain MG1655), was determined to 2.01 Å resolution. The structure, which was solved using molecular replacement, also included the structural and catalytic zinc ions and the cofactor nicotinamide adenine dinucleotide (NAD). The crystals belonged to space group P21, with unit-cell parameters a = 68.18, b = 118.92, c = 97.87 Å, β = 106.41°. The final R factor and Rfree were 0.138 and 0.184, respectively. The structure of the active site of AdhP suggested a number of residues that may participate in a proton relay, and the overall structure of AdhP, including the coordination to structural and active-site zinc ions, is similar to those of other tetrameric alcohol dehydrogenase enzymes.
AB - The crystal structure of AdhP, a recombinantly expressed alcohol dehydrogenase from Escherichia coli K-12 (substrain MG1655), was determined to 2.01 Å resolution. The structure, which was solved using molecular replacement, also included the structural and catalytic zinc ions and the cofactor nicotinamide adenine dinucleotide (NAD). The crystals belonged to space group P21, with unit-cell parameters a = 68.18, b = 118.92, c = 97.87 Å, β = 106.41°. The final R factor and Rfree were 0.138 and 0.184, respectively. The structure of the active site of AdhP suggested a number of residues that may participate in a proton relay, and the overall structure of AdhP, including the coordination to structural and active-site zinc ions, is similar to those of other tetrameric alcohol dehydrogenase enzymes.
KW - AdhP
KW - alcohol dehydrogenases
KW - Escherichia coli
UR - https://www.scopus.com/pages/publications/84880117089
U2 - 10.1107/S1744309113015170
DO - 10.1107/S1744309113015170
M3 - Article
C2 - 23832197
AN - SCOPUS:84880117089
SN - 1744-3091
VL - 69
SP - 730
EP - 732
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 7
ER -