Structure of acidic pH dengue virus showing the fusogenic glycoprotein trimers

Xinzheng Zhang, Ju Sheng, S. Kyle Austin, Tabitha E. Hoornweg, Jolanda M. Smit, Richard J. Kuhn, Michael S. Diamond, Michael G. Rossmann

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Flaviviruses undergo large conformational changes during their life cycle. Under acidic pH conditions, the mature virus forms transient fusogenic trimers of E glycoproteins that engage the lipid membrane in host cells to initiate viral fusion and nucleocapsid penetration into the cytoplasm. However, the dynamic nature of the fusogenic trimer has made the determination of its structure a challenge. Here we have used Fab fragments of the neutralizing antibody DV2-E104 to stop the conformational change of dengue virus at an intermediate stage of the fusion process. Using cryo-electron microscopy, we show that in this intermediate stage, the E glycoproteins form 60 trimers that are similar to the predicted "open" fusogenic trimer.

Original languageEnglish
Pages (from-to)743-750
Number of pages8
JournalJournal of virology
Volume89
Issue number1
DOIs
StatePublished - 2015

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