TY - JOUR
T1 - Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization
AU - Hsu, Fong Fu
AU - Turk, John
N1 - Funding Information:
This research was supported by US Public Health Service Grants P41-RR-00954, R37-DK-34388, P60-DK-20579, and P01-HL-57-278 and a grant (No. 996003) from the Juvenile Diabetes Foundation.
PY - 2000
Y1 - 2000
N2 - Alkaline metal adduct ions of sphingomyelin were formed by electrospray ionization in positive ion mode. Under low energy collisionally activated dissociation (CAD), the product ion spectra yield abundant fragment ions representative of both long chain base and fatty acid which permit unequivocal determination of the structure. Tandem spectra obtained by constant neutral loss scanning permit identification of sphingomyelin class and specific long chain base subclass in the mixture. The fragmentation pathways under CAD were proposed, and were further confirmed by source CAD tandem mass spectrometry. The total analysis of sphingomyelin mixtures from bovine brain, bovine erythrocytes, and chicken egg yolk is also presented.
AB - Alkaline metal adduct ions of sphingomyelin were formed by electrospray ionization in positive ion mode. Under low energy collisionally activated dissociation (CAD), the product ion spectra yield abundant fragment ions representative of both long chain base and fatty acid which permit unequivocal determination of the structure. Tandem spectra obtained by constant neutral loss scanning permit identification of sphingomyelin class and specific long chain base subclass in the mixture. The fragmentation pathways under CAD were proposed, and were further confirmed by source CAD tandem mass spectrometry. The total analysis of sphingomyelin mixtures from bovine brain, bovine erythrocytes, and chicken egg yolk is also presented.
UR - http://www.scopus.com/inward/record.url?scp=0034478707&partnerID=8YFLogxK
U2 - 10.1016/S1044-0305(99)00150-6
DO - 10.1016/S1044-0305(99)00150-6
M3 - Article
C2 - 10790848
AN - SCOPUS:0034478707
SN - 1044-0305
VL - 11
SP - 437
EP - 449
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 5
ER -