Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG

Parveen Goyal, Petya V. Krasteva, Nani Van Gerven, Francesca Gubellini, Imke Van Den Broeck, Anastassia Troupiotis-Tsaïlaki, Wim Jonckheere, Gérard Péhau-Arnaudet, Jerome S. Pinkner, Matthew R. Chapman, Scott J. Hultgren, Stefan Howorka, Rémi Fronzes, Han Remaut

Research output: Contribution to journalArticle

130 Scopus citations


Curli are functional amyloid fibres that constitute the major protein component of the extracellular matrix in pellicle biofilms formed by Bacteroidetes and Proteobacteria (predominantly of the α and γ 3 classes). They provide a fitness advantage in pathogenic strains and induce a strong pro-inflammatory response during bacteraemia. Curli formation requires a dedicated protein secretion machinery comprising the outer membrane lipoprotein CsgG and two soluble accessory proteins, CsgE and CsgF. Here we report the X-ray structure of Escherichia coli CsgG in a non-lipidated, soluble form as well as in its native membrane-extracted conformation. CsgG forms an oligomeric transport complex composed of nine anticodon-binding-domain-like units that give rise to a 36-stranded β 2-barrel that traverses the bilayer and is connected to a cage-like vestibule in the periplasm. The transmembrane and periplasmic domains are separated by a 0.9-nm channel constriction composed of three stacked concentric phenylalanine, asparagine and tyrosine rings that may guide the extended polypeptide substrate through the secretion pore. The specificity factor CsgE forms a nonameric adaptor that binds and closes off the periplasmic face of the secretion channel, creating a 24,000 Å 3 pre-constriction chamber. Our structural, functional and electrophysiological analyses imply that CsgG is an ungated, non-selective protein secretion channel that is expected to employ a diffusion-based, entropy-driven transport mechanism.

Original languageEnglish
Pages (from-to)250-253
Number of pages4
Issue number7530
StatePublished - Dec 11 2014

Fingerprint Dive into the research topics of 'Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG'. Together they form a unique fingerprint.

Cite this