Acute leukemia represents the most common pediatric malignancy comprising diverse sub-types with varying prognosis and treatment outcomes. New and targeted treatment op-tions are warranted for this disease. Patient-derived xenograft (PDX) models are increas-ingly being used for preclinical testing of novel treatment modalities. A novel approach in-volving targeted error-corrected RNA sequencing using ArcherDX HemeV2 kit was em-ployed to compare 25 primary pediatric acute leukemia samples and their corresponding PDX samples. A comparison of the primary samples and PDX samples revealed a high concordance between single nucleotide variants and gene fusions whereas other complex structural variants were not as consistent. The presence of gene fusions representing the major driver mutations at similar allelic frequencies in PDX samples compared to primary samples and over multiple passages confirms the utility of PDX models for preclinical drug testing. Characterization and tracking of these novel cryptic fusions and exonal variants in PDX models is critical in assessing response to potential new therapies.
|Journal||Genomics and Informatics|
|State||Published - Mar 2020|
- Error-corrected sequencing
- Patient derived xenograft models
- Pediatric cancers
- Structural variants