Stress response genes protect against lethal effects of sleep deprivation in Drosophila

Paul J. Shaw, Giulio Tortoni, Ralph J. Greenspan, Donald F. Robinson

Research output: Contribution to journalArticle

317 Scopus citations


Sleep is controlled by two processes: a homeostatic drive that increases during waking and dissipates during sleep, and a circadian pacemaker that controls its timing. Although these two systems can operate independently, recent studies indicate a more intimate relationship. To study the interaction between homeostatic and circadian processes in Drosophila, we examined homeostasis in the canonical loss-of-function clock mutants period (per01), timeless (tim01), clock (Clkjrk) and cycle (cyc01). cyc01 mutants showed a disproportionately large sleep rebound and died after 10 hours of sleep deprivation, although they were more resistant than other clock mutants to various stressors. Unlike other clock mutants, cyc01 flies showed a reduced expression of heat-shock genes after sleep loss. However, activating heat-shock genes before sleep deprivation rescued cyc01 flies from its lethal effects. Consistent with the protective effect of heat-shock genes, was the observation that flies carrying a mutation for the heat-shock protein Hsp83 (Hsp8308445) showed exaggerated homeostatic response and died after sleep deprivation. These data represent the first step in identifying the molecular mechanisms that constitute the sleep homeostat.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
Issue number6886
StatePublished - May 16 2002
Externally publishedYes

Fingerprint Dive into the research topics of 'Stress response genes protect against lethal effects of sleep deprivation in Drosophila'. Together they form a unique fingerprint.

  • Cite this