TY - JOUR
T1 - Strcuture and transcription of the nopaline synthase gene region of T-DNA
AU - Bevan, Michael
AU - Barnes, Wayne M.
AU - Chilton, Marry dell
N1 - Funding Information:
ACKHOMLZDCEHEHIS The authors wish to thank Elodee Tuley, Don Bennett and Jim Grichnik for their able assistance. This research was supported by the NIH (grant no. GM24956 to W.M.B.), the American Cancer Society (Faculty Research Award to W.H.B. and grant NP194 to M.D.C.), the Department of Energy (contract AC02-81ER 10889, and Monsanto Corporation (postdoctoral fellowship to H.B.).
PY - 1983/1/25
Y1 - 1983/1/25
N2 - We present the DNA sequence and plant-tumor transcription pattern of some 2400 base pairs from the right border region of pTi T37 DNA from the virulent Agrobacterium tumefaciens strain T37. This region includes the entire transcription unit encompassing the nopaline synthase gene, together with parts of other transcription units. The strategy used to determine the sequence also produced two opposing series of defined, asymmetric deletions across the target DNA region, some of which may serve future purposes in the exploitation of this sequence, which is known to be expressed in a wide variety of host plant tissues.
AB - We present the DNA sequence and plant-tumor transcription pattern of some 2400 base pairs from the right border region of pTi T37 DNA from the virulent Agrobacterium tumefaciens strain T37. This region includes the entire transcription unit encompassing the nopaline synthase gene, together with parts of other transcription units. The strategy used to determine the sequence also produced two opposing series of defined, asymmetric deletions across the target DNA region, some of which may serve future purposes in the exploitation of this sequence, which is known to be expressed in a wide variety of host plant tissues.
UR - http://www.scopus.com/inward/record.url?scp=0021111520&partnerID=8YFLogxK
U2 - 10.1093/nar/11.2.369
DO - 10.1093/nar/11.2.369
M3 - Article
C2 - 6298724
AN - SCOPUS:0021111520
VL - 11
SP - 369
EP - 385
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 2
ER -