Strcuture and transcription of the nopaline synthase gene region of T-DNA

Michael Bevan, Wayne M. Barnes, Marry dell Chilton

Research output: Contribution to journalArticle

229 Scopus citations

Abstract

We present the DNA sequence and plant-tumor transcription pattern of some 2400 base pairs from the right border region of pTi T37 DNA from the virulent Agrobacterium tumefaciens strain T37. This region includes the entire transcription unit encompassing the nopaline synthase gene, together with parts of other transcription units. The strategy used to determine the sequence also produced two opposing series of defined, asymmetric deletions across the target DNA region, some of which may serve future purposes in the exploitation of this sequence, which is known to be expressed in a wide variety of host plant tissues.

Original languageEnglish
Pages (from-to)369-385
Number of pages17
JournalNucleic acids research
Volume11
Issue number2
DOIs
StatePublished - Jan 25 1983

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