Strain-specific susceptibility for pulmonary metastasis of sarcoma 180 cells in inbred mice

Haris G. Vikis, Erin N. Jackson, Alexander S. Krupnick, Andrew Franklin, Andrew E. Gelman, Qiong Chen, David Piwnica-Worms, Ming You

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Most cancer deaths are a result of metastasis. To extend our understanding of the factors that influence the process, we aimed to develop a mouse model of pulmonary metastasis that can be assayed in multiple inbred mouse strains for further use in identification of host genetic variants that influence metastasis. We used i.v. injection of Sarcoma 180 (S180) cells, which can be tracked and quantified by bioluminescence imaging. We observed growth of S180 cells solely in the lung and observed a wide range of pulmonary metastasis among inbred mouse strains. Interestingly, we noted that the BTBRT+tf/J strain exhibited complete clearance and provide evidence that the mechanism of resistance may involve immune factors, as strains subjected to whole-body irradiation are significantly more susceptible to tumor growth. One possible mechanism of resistance to pulmonary metastasis in BTBRT+tf/J mice may require T-cell function. Our experiments present a new mouse model for further characterization of the genetics and mechanisms of pulmonary metastasis.

Original languageEnglish
Pages (from-to)4859-4867
Number of pages9
JournalCancer research
Volume70
Issue number12
DOIs
StatePublished - Jun 15 2010

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