Strain-dependent fluorescence correlation spectroscopy: Proposing a new measurement for conformational fluctuations of biological macromolecules

Hong Qian, Elliot L. Elson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have proposed a measurement in which a single protein or nucleic acid molecule is held by an externally applied tension in a partially folded intermediate state. In this state it could be possible to observe the dynamics of conformational changes that would be obscured by conformational cooperativity in conventional experiments, We have presented a simple model that illustrates how this approach might work to reveal states, which could not readily be observed in the absence of the applied force. This measurement approach is applicable to any macromolecular system and could be especially useful in analyzing the processes that dominate protein folding kinetic studies of which began three decades ago [4.30].

Original languageEnglish
Pages (from-to)65-83
Number of pages19
JournalSpringer Series in Chemical Physics
Volume65
DOIs
StatePublished - Jan 1 2001

Fingerprint

Dive into the research topics of 'Strain-dependent fluorescence correlation spectroscopy: Proposing a new measurement for conformational fluctuations of biological macromolecules'. Together they form a unique fingerprint.

Cite this