Stimulation of purified phosphatidylinositol 4-kinase by cobra venom cardiotoxin

Duncan H. Walker, Linda J. Pike

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Cobra venom cardiotoxin was found to stimulate the phosphatidylinositol kinase activity present in A431 cell membranes and in detergent extracts of these membranes. Incubation of highly purified phosphatidylinositol 4-kinase from this source with cardiotoxin resulted in a 2- to 3-fold stimulation of phosphatidylinositol kinase activity. The activation of the purified phosphatidylinositol 4-kinase by cardiotoxin was time- and dose-dependent and appeared to be associated with a decrease in the Km apparent of the enzyme for phosphatidylinositol with no change in the Vmax apparent of the enzyme. The data suggest that the phosphatidylinositol 4-kinase is activated by direct interaction of the enzyme with cobra venom cardiotoxin.

Original languageEnglish
Pages (from-to)295-298
Number of pages4
JournalBBA - Molecular Cell Research
Volume1055
Issue number3
DOIs
StatePublished - Dec 10 1990

Keywords

  • (A431 cell)
  • Cardiotoxin
  • Phosphatidylinositol kinase

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