Abstract
Cobra venom cardiotoxin was found to stimulate the phosphatidylinositol kinase activity present in A431 cell membranes and in detergent extracts of these membranes. Incubation of highly purified phosphatidylinositol 4-kinase from this source with cardiotoxin resulted in a 2- to 3-fold stimulation of phosphatidylinositol kinase activity. The activation of the purified phosphatidylinositol 4-kinase by cardiotoxin was time- and dose-dependent and appeared to be associated with a decrease in the Km apparent of the enzyme for phosphatidylinositol with no change in the Vmax apparent of the enzyme. The data suggest that the phosphatidylinositol 4-kinase is activated by direct interaction of the enzyme with cobra venom cardiotoxin.
Original language | English |
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Pages (from-to) | 295-298 |
Number of pages | 4 |
Journal | BBA - Molecular Cell Research |
Volume | 1055 |
Issue number | 3 |
DOIs | |
State | Published - Dec 10 1990 |
Keywords
- (A431 cell)
- Cardiotoxin
- Phosphatidylinositol kinase