Stimulation of purified phosphatidylinositol 4-kinase by cobra venom cardiotoxin

Duncan H. Walker, Linda J. Pike

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Cobra venom cardiotoxin was found to stimulate the phosphatidylinositol kinase activity present in A431 cell membranes and in detergent extracts of these membranes. Incubation of highly purified phosphatidylinositol 4-kinase from this source with cardiotoxin resulted in a 2- to 3-fold stimulation of phosphatidylinositol kinase activity. The activation of the purified phosphatidylinositol 4-kinase by cardiotoxin was time- and dose-dependent and appeared to be associated with a decrease in the Km apparent of the enzyme for phosphatidylinositol with no change in the Vmax apparent of the enzyme. The data suggest that the phosphatidylinositol 4-kinase is activated by direct interaction of the enzyme with cobra venom cardiotoxin.

Original languageEnglish
Pages (from-to)295-298
Number of pages4
JournalBBA - Molecular Cell Research
Issue number3
StatePublished - Dec 10 1990


  • (A431 cell)
  • Cardiotoxin
  • Phosphatidylinositol kinase


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