Stem cell expression of the AML1/ETO fusion protein induces a myeloproliferative disorder in mice

Timothy S. Fenske, Gina Pengue, Vikram Mathews, Piia T. Hanson, Sarah E. Hamm, Moor Riaz, Timothy A. Graubert

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

The t(8;21)(q22;q22) translocation, present in 10-15% of acute myeloid leukemia (AML) cases, generates the AML1/ETO fusion protein. To study the role of AML1/ETO in the pathogenesis of AML, we used the Ly6A locus that encodes the well characterized hematopoietic stem cell marker. Sca1, to target expression of AML1/ETO to the hamatopoietic stem cell compartment in mice. Whereas germ-line expression of AML1/ETO from the AML1 promoter results in embryonic lethality, heterozygous sca1+/AML1/ETO ires EGFP (abbreviated Sca +/AE) mutant mice are born in Mendelian ratios with no apparent abnormalities in growth or fertility. Hematopoietic cells from Sca +/AE mice have markedly extended survival in vitro and increasing myeloid clonogenic progenitor output over time. Sca+/AE mice develop a spontaneous myeloproliferative disorder with a latency of 6 months and a penetrance of 82% at 14 months. These results reinforce the notion that the phenotype of murine transgenic models of human leukemia is critically dependent on the cellular compartment targeted by the transgene. This model should provide a useful platform to analyze the effect of AML1/ETO on hematopoiesis and its potential cooperation with other mutations in the pathogenesis of leukemia.

Original languageEnglish
Pages (from-to)15184-15189
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number42
DOIs
StatePublished - Oct 19 2004

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