STED nanoscopy in living cells using fluorogen activating proteins

James A.J. Fitzpatrick; Qi Yan; Jochen J. Sieber; Marcus Dyba; Ulf Schwarz; Chris Szent-Gyorgyi; Carol A. Woolford; Peter B. Berget; Alan S. Waggoner; Marcel P. Bruchez

doi:10.1021/bc900249e

STED nanoscopy in living cells using fluorogen activating proteins

James A.J. Fitzpatrick, Qi Yan, Jochen J. Sieber, Marcus Dyba, Ulf Schwarz, Chris Szent-Gyorgyi, Carol A. Woolford, Peter B. Berget, Alan S. Waggoner, Marcel P. Bruchez

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69 Scopus citations

Abstract

We demonstrate the effectiveness of a genetically encoded malachite green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with widths of 110-122 nm were observed. Depletion data, however, suggest a resolution of 70 nm with the given instrument.

Original language	English
Pages (from-to)	1843-1847
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	20
Issue number	10
DOIs	https://doi.org/10.1021/bc900249e
State	Published - Oct 21 2009

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abstract = "We demonstrate the effectiveness of a genetically encoded malachite green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with widths of 110-122 nm were observed. Depletion data, however, suggest a resolution of 70 nm with the given instrument.",

author = "Fitzpatrick, {James A.J.} and Qi Yan and Sieber, {Jochen J.} and Marcus Dyba and Ulf Schwarz and Chris Szent-Gyorgyi and Woolford, {Carol A.} and Berget, {Peter B.} and Waggoner, {Alan S.} and Bruchez, {Marcel P.}",

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T1 - STED nanoscopy in living cells using fluorogen activating proteins

AU - Fitzpatrick, James A.J.

AU - Yan, Qi

AU - Sieber, Jochen J.

AU - Dyba, Marcus

AU - Schwarz, Ulf

AU - Szent-Gyorgyi, Chris

AU - Woolford, Carol A.

AU - Berget, Peter B.

AU - Waggoner, Alan S.

AU - Bruchez, Marcel P.

PY - 2009/10/21

Y1 - 2009/10/21

N2 - We demonstrate the effectiveness of a genetically encoded malachite green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with widths of 110-122 nm were observed. Depletion data, however, suggest a resolution of 70 nm with the given instrument.

AB - We demonstrate the effectiveness of a genetically encoded malachite green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with widths of 110-122 nm were observed. Depletion data, however, suggest a resolution of 70 nm with the given instrument.

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DO - 10.1021/bc900249e

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VL - 20

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JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 10

ER -

STED nanoscopy in living cells using fluorogen activating proteins

Abstract

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