Standardized two-step testing of antibody activity in COVID-19 convalescent plasma

Pavlo Gilchuk; Isaac Thomsen; Sandra Yoder; Eric Brady; James D. Chappell; Laura J. Stevens; Mark R. Denison; Rachel E. Sutton; Rita E. Chen; Laura A. VanBlargan; Naveenchandra Suryadevara; Seth J. Zost; Jonathan Schmitz; Jill M. Pulley; Michael S. Diamond; Jillian P. Rhoads; Gordon R. Bernard; Wesley H. Self; Todd W. Rice; Allison P. Wheeler; James E. Crowe; Robert H. Carnahan

doi:10.1016/j.isci.2021.103602

Standardized two-step testing of antibody activity in COVID-19 convalescent plasma

Pavlo Gilchuk, Isaac Thomsen, Sandra Yoder, Eric Brady, James D. Chappell, Laura J. Stevens, Mark R. Denison, Rachel E. Sutton, Rita E. Chen, Laura A. VanBlargan, Naveenchandra Suryadevara, Seth J. Zost, Jonathan Schmitz, Jill M. Pulley, Michael S. Diamond, Jillian P. Rhoads, Gordon R. Bernard, Wesley H. Self, Todd W. Rice, Allison P. WheelerJames E. Crowe, Robert H. Carnahan

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5 Scopus citations

Abstract

The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.

Original language	English
Article number	103602
Journal	iScience
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/j.isci.2021.103602
State	Published - Jan 21 2022

Keywords

Immunology
Virology

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10.1016/j.isci.2021.103602

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Gilchuk, P., Thomsen, I., Yoder, S., Brady, E., Chappell, J. D., Stevens, L. J., Denison, M. R., Sutton, R. E., Chen, R. E., VanBlargan, L. A., Suryadevara, N., Zost, S. J., Schmitz, J., Pulley, J. M., Diamond, M. S., Rhoads, J. P., Bernard, G. R., Self, W. H., Rice, T. W., ... Carnahan, R. H. (2022). Standardized two-step testing of antibody activity in COVID-19 convalescent plasma. iScience, 25(1), Article 103602. https://doi.org/10.1016/j.isci.2021.103602

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title = "Standardized two-step testing of antibody activity in COVID-19 convalescent plasma",

abstract = "The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.",

keywords = "Immunology, Virology",

author = "Pavlo Gilchuk and Isaac Thomsen and Sandra Yoder and Eric Brady and Chappell, {James D.} and Stevens, {Laura J.} and Denison, {Mark R.} and Sutton, {Rachel E.} and Chen, {Rita E.} and VanBlargan, {Laura A.} and Naveenchandra Suryadevara and Zost, {Seth J.} and Jonathan Schmitz and Pulley, {Jill M.} and Diamond, {Michael S.} and Rhoads, {Jillian P.} and Bernard, {Gordon R.} and Self, {Wesley H.} and Rice, {Todd W.} and Wheeler, {Allison P.} and Crowe, {James E.} and Carnahan, {Robert H.}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2022",

month = jan,

day = "21",

doi = "10.1016/j.isci.2021.103602",

language = "English",

volume = "25",

journal = "iScience",

issn = "2589-0042",

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Gilchuk, P, Thomsen, I, Yoder, S, Brady, E, Chappell, JD, Stevens, LJ, Denison, MR, Sutton, RE, Chen, RE, VanBlargan, LA, Suryadevara, N, Zost, SJ, Schmitz, J, Pulley, JM, Diamond, MS, Rhoads, JP, Bernard, GR, Self, WH, Rice, TW, Wheeler, AP, Crowe, JE & Carnahan, RH 2022, 'Standardized two-step testing of antibody activity in COVID-19 convalescent plasma', iScience, vol. 25, no. 1, 103602. https://doi.org/10.1016/j.isci.2021.103602

TY - JOUR

T1 - Standardized two-step testing of antibody activity in COVID-19 convalescent plasma

AU - Gilchuk, Pavlo

AU - Thomsen, Isaac

AU - Yoder, Sandra

AU - Brady, Eric

AU - Chappell, James D.

AU - Stevens, Laura J.

AU - Denison, Mark R.

AU - Sutton, Rachel E.

AU - Chen, Rita E.

AU - VanBlargan, Laura A.

AU - Suryadevara, Naveenchandra

AU - Zost, Seth J.

AU - Schmitz, Jonathan

AU - Pulley, Jill M.

AU - Diamond, Michael S.

AU - Rhoads, Jillian P.

AU - Bernard, Gordon R.

AU - Self, Wesley H.

AU - Rice, Todd W.

AU - Wheeler, Allison P.

AU - Crowe, James E.

AU - Carnahan, Robert H.

PY - 2022/1/21

Y1 - 2022/1/21

N2 - The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.

AB - The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.

KW - Immunology

KW - Virology

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U2 - 10.1016/j.isci.2021.103602

DO - 10.1016/j.isci.2021.103602

M3 - Article

C2 - 34901783

AN - SCOPUS:85121625428

SN - 2589-0042

VL - 25

JO - iScience

JF - iScience

IS - 1

M1 - 103602

ER -

Standardized two-step testing of antibody activity in COVID-19 convalescent plasma

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Keywords

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