TY - JOUR
T1 - Stability of varicella-zoster virus open reading frame 63
AU - Liu, Merry
AU - Vafai, Nicholas
AU - Liu, Angela
AU - Hart, John
AU - Liu, Hsi
AU - He, Ju
AU - Tang, Xiaoling
AU - Wang, Dongxia
AU - Vafai, Abbas
PY - 2008/10
Y1 - 2008/10
N2 - The stability of varicella-zoster virus (VZV) open reading frame (ORF) 63 was analyzed by sequential passage of a virus strain in cell culture. VZV was propagated in culture for 1,206 passages. ORF63 from six passages (18, 220, 516, 730, 1060, and 1,206) was selected and sequenced. Among the six passages, only passage 1,206 showed point mutations at three locations: 551, 618 and 661. In addition, western blot analysis with anti-ORF63 monoclonal antibodies showed no discernable difference in the size of the ORF63 gene product from passage 18 and that from passage 1,206. These results indicate the stability of VZV ORF63 gene in culture over 1,206 passages.
AB - The stability of varicella-zoster virus (VZV) open reading frame (ORF) 63 was analyzed by sequential passage of a virus strain in cell culture. VZV was propagated in culture for 1,206 passages. ORF63 from six passages (18, 220, 516, 730, 1060, and 1,206) was selected and sequenced. Among the six passages, only passage 1,206 showed point mutations at three locations: 551, 618 and 661. In addition, western blot analysis with anti-ORF63 monoclonal antibodies showed no discernable difference in the size of the ORF63 gene product from passage 18 and that from passage 1,206. These results indicate the stability of VZV ORF63 gene in culture over 1,206 passages.
UR - http://www.scopus.com/inward/record.url?scp=53849137992&partnerID=8YFLogxK
U2 - 10.1007/s00705-008-0197-4
DO - 10.1007/s00705-008-0197-4
M3 - Article
C2 - 18807114
AN - SCOPUS:53849137992
SN - 0304-8608
VL - 153
SP - 1943
EP - 1947
JO - Archives of Virology
JF - Archives of Virology
IS - 10
ER -