Stability of varicella-zoster virus open reading frame 63

Merry Liu, Nicholas Vafai, Angela Liu, John Hart, Hsi Liu, Ju He, Xiaoling Tang, Dongxia Wang, Abbas Vafai

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The stability of varicella-zoster virus (VZV) open reading frame (ORF) 63 was analyzed by sequential passage of a virus strain in cell culture. VZV was propagated in culture for 1,206 passages. ORF63 from six passages (18, 220, 516, 730, 1060, and 1,206) was selected and sequenced. Among the six passages, only passage 1,206 showed point mutations at three locations: 551, 618 and 661. In addition, western blot analysis with anti-ORF63 monoclonal antibodies showed no discernable difference in the size of the ORF63 gene product from passage 18 and that from passage 1,206. These results indicate the stability of VZV ORF63 gene in culture over 1,206 passages.

Original languageEnglish
Pages (from-to)1943-1947
Number of pages5
JournalArchives of Virology
Volume153
Issue number10
DOIs
StatePublished - Oct 2008

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