TY - JOUR
T1 - SSB as an organizer/mobilizer of genome maintenance complexes
AU - Shereda, Robert D.
AU - Kozlov, Alexander G.
AU - Lohman, Timothy M.
AU - Cox, Michael M.
AU - Keck, James L.
N1 - Funding Information:
J.L.K acknowledges financial support from the NIH (GM068061). T.M.L. acknowledges financial support from the NIH (GM030498). M.M.C. acknowledges financial support from the NIH (GM032335 and GM0676085). R.D.S. is a Cremer Scholar. We apologize to any colleagues whose contributions to SSB studies might have been inadvertently overlooked in this review.
PY - 2008/9
Y1 - 2008/9
N2 - When duplex DNA is altered in almost any way (replicated, recombined, or repaired), single strands of DNA are usually intermediates, and single-stranded DNA binding (SSB) proteins are present. These proteins have often been described as inert, protective DNA coatings. Continuing research is demonstrating a far more complex role of SSB that includes the organization and/or mobilization of all aspects of DNA metabolism. Escherichia coli SSB is now known to interact with at least 14 other proteins that include key components of the elaborate systems involved in every aspect of DNA metabolism. Most, if not all, of these interactions are mediated by the amphipathic C-terminus of SSB. In this review, we summarize the extent of the eubacterial SSB interaction network, describe the energetics of interactions with SSB, and highlight the roles of SSB in the process of recombination. Similar themes to those highlighted in this review are evident in all biological systems.
AB - When duplex DNA is altered in almost any way (replicated, recombined, or repaired), single strands of DNA are usually intermediates, and single-stranded DNA binding (SSB) proteins are present. These proteins have often been described as inert, protective DNA coatings. Continuing research is demonstrating a far more complex role of SSB that includes the organization and/or mobilization of all aspects of DNA metabolism. Escherichia coli SSB is now known to interact with at least 14 other proteins that include key components of the elaborate systems involved in every aspect of DNA metabolism. Most, if not all, of these interactions are mediated by the amphipathic C-terminus of SSB. In this review, we summarize the extent of the eubacterial SSB interaction network, describe the energetics of interactions with SSB, and highlight the roles of SSB in the process of recombination. Similar themes to those highlighted in this review are evident in all biological systems.
UR - http://www.scopus.com/inward/record.url?scp=54349118941&partnerID=8YFLogxK
U2 - 10.1080/10409230802341296
DO - 10.1080/10409230802341296
M3 - Review article
C2 - 18937104
AN - SCOPUS:54349118941
SN - 1040-9238
VL - 43
SP - 289
EP - 318
JO - Critical Reviews in Biochemistry and Molecular Biology
JF - Critical Reviews in Biochemistry and Molecular Biology
IS - 5
ER -