Src phosphorylation of cortactin enhances actin assembly

Shandiz Tehrani; Nenad Tomasevic; Scott Weed; Roman Sakowicz; John A. Cooper

doi:10.1073/pnas.0701077104

Src phosphorylation of cortactin enhances actin assembly

Shandiz Tehrani
, Nenad Tomasevic
, Scott Weed
, Roman Sakowicz
, John A. Cooper

Research output: Contribution to journal › Article › peer-review

206 Scopus citations

Abstract

Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.

Original language	English
Pages (from-to)	11933-11938
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	29
DOIs	https://doi.org/10.1073/pnas.0701077104
State	Published - Jul 17 2007

Keywords

N-WASp
Nck
Tyrosine phosphorylation

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10.1073/pnas.0701077104

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title = "Src phosphorylation of cortactin enhances actin assembly",

abstract = "Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.",

keywords = "N-WASp, Nck, Tyrosine phosphorylation",

author = "Shandiz Tehrani and Nenad Tomasevic and Scott Weed and Roman Sakowicz and Cooper, \{John A.\}",

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doi = "10.1073/pnas.0701077104",

language = "English",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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T1 - Src phosphorylation of cortactin enhances actin assembly

AU - Tehrani, Shandiz

AU - Tomasevic, Nenad

AU - Weed, Scott

AU - Sakowicz, Roman

AU - Cooper, John A.

PY - 2007/7/17

Y1 - 2007/7/17

N2 - Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.

AB - Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.

KW - N-WASp

KW - Nck

KW - Tyrosine phosphorylation

UR - https://www.scopus.com/pages/publications/34547513265

U2 - 10.1073/pnas.0701077104

DO - 10.1073/pnas.0701077104

M3 - Article

C2 - 17606906

AN - SCOPUS:34547513265

SN - 0027-8424

VL - 104

SP - 11933

EP - 11938

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 29

ER -

Src phosphorylation of cortactin enhances actin assembly

Abstract

Keywords

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