TY - JOUR
T1 - Src phosphorylation of cortactin enhances actin assembly
AU - Tehrani, Shandiz
AU - Tomasevic, Nenad
AU - Weed, Scott
AU - Sakowicz, Roman
AU - Cooper, John A.
PY - 2007/7/17
Y1 - 2007/7/17
N2 - Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.
AB - Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, and motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance actin assembly is unknown. Here, using an actin assembly system reconstituted from purified components, we demonstrate for the first time a biochemical mechanism by which Src phosphorylation of cortactin affects actin assembly. The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex.
KW - N-WASp
KW - Nck
KW - Tyrosine phosphorylation
UR - http://www.scopus.com/inward/record.url?scp=34547513265&partnerID=8YFLogxK
U2 - 10.1073/pnas.0701077104
DO - 10.1073/pnas.0701077104
M3 - Article
C2 - 17606906
AN - SCOPUS:34547513265
SN - 0027-8424
VL - 104
SP - 11933
EP - 11938
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 29
ER -