Spontaneous Activity in the Zebrafish Tectum Reorganizes over Development and Is Influenced by Visual Experience

Lilach Avitan; Zac Pujic; Jan Mölter; Matthew Van De Poll; Biao Sun; Haotian Teng; Rumelo Amor; Ethan K. Scott; Geoffrey J. Goodhill

doi:10.1016/j.cub.2017.06.056

Spontaneous Activity in the Zebrafish Tectum Reorganizes over Development and Is Influenced by Visual Experience

Lilach Avitan, Zac Pujic, Jan Mölter, Matthew Van De Poll, Biao Sun, Haotian Teng, Rumelo Amor, Ethan K. Scott, Geoffrey J. Goodhill

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Spontaneous patterns of activity in the developing visual system may play an important role in shaping the brain for function. During the period 4–9 dpf (days post-fertilization), larval zebrafish learn to hunt prey, a behavior that is critically dependent on the optic tectum. However, how spontaneous activity develops in the tectum over this period and the effect of visual experience are unknown. Here we performed two-photon calcium imaging of GCaMP6s zebrafish larvae at all days from 4 to 9 dpf. Using recently developed graph theoretic techniques, we found significant changes in both single-cell and population activity characteristics over development. In particular, we identified days 5–6 as a critical moment in the reorganization of the underlying functional network. Altering visual experience early in development altered the statistics of tectal activity, and dark rearing also caused a long-lasting deficit in the ability to capture prey. Thus, tectal development is shaped by both intrinsic factors and visual experience.

Original language	English
Pages (from-to)	2407-2419.e4
Journal	Current Biology
Volume	27
Issue number	16
DOIs	https://doi.org/10.1016/j.cub.2017.06.056
State	Published - Aug 21 2017

Keywords

behavior
calcium imaging
critical period
dark rearing
graph theory
neural assemblies
neural coding
neural plasticity
prey capture
visual development

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10.1016/j.cub.2017.06.056

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title = "Spontaneous Activity in the Zebrafish Tectum Reorganizes over Development and Is Influenced by Visual Experience",

abstract = "Spontaneous patterns of activity in the developing visual system may play an important role in shaping the brain for function. During the period 4–9 dpf (days post-fertilization), larval zebrafish learn to hunt prey, a behavior that is critically dependent on the optic tectum. However, how spontaneous activity develops in the tectum over this period and the effect of visual experience are unknown. Here we performed two-photon calcium imaging of GCaMP6s zebrafish larvae at all days from 4 to 9 dpf. Using recently developed graph theoretic techniques, we found significant changes in both single-cell and population activity characteristics over development. In particular, we identified days 5–6 as a critical moment in the reorganization of the underlying functional network. Altering visual experience early in development altered the statistics of tectal activity, and dark rearing also caused a long-lasting deficit in the ability to capture prey. Thus, tectal development is shaped by both intrinsic factors and visual experience.",

keywords = "behavior, calcium imaging, critical period, dark rearing, graph theory, neural assemblies, neural coding, neural plasticity, prey capture, visual development",

author = "Lilach Avitan and Zac Pujic and Jan M{\"o}lter and {Van De Poll}, Matthew and Biao Sun and Haotian Teng and Rumelo Amor and Scott, {Ethan K.} and Goodhill, {Geoffrey J.}",

note = "Funding Information: We thank Misha Ahrens for the elavl3:H2B:GCaMP6s transgenic line. This work was funded by the Australian Research Council (grant DP150101152). Imaging was performed at the Queensland Brain Institute's Advanced Microscopy Facility and funded by the Australian Government through the ARC LIEF grant LE130100078. Funding Information: We thank Misha Ahrens for the elavl3:H2B:GCaMP6s transgenic line. This work was funded by the Australian Research Council (grant DP150101152 ). Imaging was performed at the Queensland Brain Institute{\textquoteright}s Advanced Microscopy Facility and funded by the Australian Government through the ARC LIEF grant LE130100078 . Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

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doi = "10.1016/j.cub.2017.06.056",

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AU - Avitan, Lilach

AU - Pujic, Zac

AU - Mölter, Jan

AU - Van De Poll, Matthew

AU - Sun, Biao

AU - Teng, Haotian

AU - Amor, Rumelo

AU - Scott, Ethan K.

AU - Goodhill, Geoffrey J.

N1 - Funding Information: We thank Misha Ahrens for the elavl3:H2B:GCaMP6s transgenic line. This work was funded by the Australian Research Council (grant DP150101152). Imaging was performed at the Queensland Brain Institute's Advanced Microscopy Facility and funded by the Australian Government through the ARC LIEF grant LE130100078. Funding Information: We thank Misha Ahrens for the elavl3:H2B:GCaMP6s transgenic line. This work was funded by the Australian Research Council (grant DP150101152 ). Imaging was performed at the Queensland Brain Institute’s Advanced Microscopy Facility and funded by the Australian Government through the ARC LIEF grant LE130100078 . Publisher Copyright: © 2017 Elsevier Ltd

PY - 2017/8/21

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N2 - Spontaneous patterns of activity in the developing visual system may play an important role in shaping the brain for function. During the period 4–9 dpf (days post-fertilization), larval zebrafish learn to hunt prey, a behavior that is critically dependent on the optic tectum. However, how spontaneous activity develops in the tectum over this period and the effect of visual experience are unknown. Here we performed two-photon calcium imaging of GCaMP6s zebrafish larvae at all days from 4 to 9 dpf. Using recently developed graph theoretic techniques, we found significant changes in both single-cell and population activity characteristics over development. In particular, we identified days 5–6 as a critical moment in the reorganization of the underlying functional network. Altering visual experience early in development altered the statistics of tectal activity, and dark rearing also caused a long-lasting deficit in the ability to capture prey. Thus, tectal development is shaped by both intrinsic factors and visual experience.

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KW - graph theory

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JO - Current Biology

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ER -

Spontaneous Activity in the Zebrafish Tectum Reorganizes over Development and Is Influenced by Visual Experience

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Keywords

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