Several methods of damaging red blood cells (RBCs) for splenic imaging were compared to determine the optimum approach. The RBCs from donor animals were labeled with (99m)TcO4- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N ethylmaleimide (NEM) or p hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBCs were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen to liver ratios with heat or sulfhydryl damaged (99m)Tc RBCs were significantly greater (p <0.001) than the values obtained using ACD or Sn(II) ion. Heat damaging produces good splenic localization of (99m)Tc RBCs but requires rigidly controlled incubation conditions. NEM damaging provides an excellent and predictable alternative approach.
|Number of pages||6|
|Journal||Journal of Nuclear Medicine|
|State||Published - Jan 1 1976|