Spectrin localization in osteoclasts: Immunocytochemistry, cloning, and partial sequencing

Susan J. Hunter, Carol V. Gay, Philip A. Osdoby, Luanne L. Peters

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The presence of spectrin was demonstrated in chick osteoclasts by Western blotting and light and electron microscopic: immunolocalization. Additionally, screening of a chick osteoclast cDNA library revealed the presence of α-spectrin. Light microscope level immunocytochemical staining of osteoclasts in situ revealed spectrin staining throughout the cytoplasm with heavier staining found at the marrow-facing cell margin and around the nuclei. Confocal microscopy of isolated osteoclasts plated onto a glass substrate showed that spectrin encircled the organelle-rich cell center. Nuclei and cytoplasmic inclusions were also stained and the plasma membrane was stained in a nonuniform, patchy distribution corresponding to regions of apparent membrane ruffling. Ultracytochemical localization showed spectrin to be found at the plasma membrane and distributed throughout the cytoplasm with especially intense staining of the nuclear membrane and filaments within the nuclear compartment.

Original languageEnglish
Pages (from-to)204-215
Number of pages12
JournalJournal of cellular biochemistry
Issue number2
StatePublished - Nov 1 1998


  • Bone
  • Bone resorption
  • Membrane skeleton
  • Osteoclast
  • Spectrin


Dive into the research topics of 'Spectrin localization in osteoclasts: Immunocytochemistry, cloning, and partial sequencing'. Together they form a unique fingerprint.

Cite this