TY - JOUR
T1 - Specific T cell recognition of minimally homologous peptides
T2 - Evidence for multiple endogenous Ligands
AU - Evavold, Brian D.
AU - Sloan-Lancastert, Joanne
AU - Wilson, K. Jeff
AU - Rothbard, Jonathan B.
AU - Allen, Paul M.
N1 - Funding Information:
We thank D. Donermeyer for technical assistance. We also thank E. Unanue, R. Schreiber, P. Jensen, and M. Holsti for their comments and advice. This work was supported by grants from the American Cancer Society and the National Institutes of Health.
PY - 1995/6
Y1 - 1995/6
N2 - The T cell receptor (TCR) can interact with a spectrum of peptides as part of its ligand, Including the immunogenic peptide, variants of this peptide, and apparently unrelated peptides. The basis of this broad specificity for ligand was investigated by substitution analysis of a peptide antigen and functional testing using a B cell apoptosls assay. A peptide containing as few as 1 aa in common with this peptide could stimulate a specific T cell response. Two endogenous ligands, an agonist and a partial agonist, were readily Identified from a search of the SwissProt database, indicating that multiple endogenous ligands likely exist for a given T cell. These findings strongly support the concept that one TCR has the ability to interact productively with multiple different Iigands, and provide evidence that such ligands exist in the endogenous peptide repertoire.
AB - The T cell receptor (TCR) can interact with a spectrum of peptides as part of its ligand, Including the immunogenic peptide, variants of this peptide, and apparently unrelated peptides. The basis of this broad specificity for ligand was investigated by substitution analysis of a peptide antigen and functional testing using a B cell apoptosls assay. A peptide containing as few as 1 aa in common with this peptide could stimulate a specific T cell response. Two endogenous ligands, an agonist and a partial agonist, were readily Identified from a search of the SwissProt database, indicating that multiple endogenous ligands likely exist for a given T cell. These findings strongly support the concept that one TCR has the ability to interact productively with multiple different Iigands, and provide evidence that such ligands exist in the endogenous peptide repertoire.
UR - http://www.scopus.com/inward/record.url?scp=0028979705&partnerID=8YFLogxK
U2 - 10.1016/1074-7613(95)90010-1
DO - 10.1016/1074-7613(95)90010-1
M3 - Article
C2 - 7540944
AN - SCOPUS:0028979705
SN - 1074-7613
VL - 2
SP - 655
EP - 663
JO - Immunity
JF - Immunity
IS - 6
ER -