Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast

Christine Sütterlin, Tamara L. Doering, Frauke Schimmöller, Stephan Schröder, Howard Riezman

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147 Scopus citations

Abstract

GPI-anchored proteins are attached to the membrane via a glycosylphosphatidylinositol-(GPI) anchor whose carbohydrate core is conserved in all eukaryotes. Apart from membrane attachment, the precise role of the GPI-anchor is not known, but it has been proposed to play a role in protein sorting. We have investigated the transport of the yeast GPI-anchored protein Gas1p. We identified two mutant strains involved in very different cellular processes that are blocked selectively in the transport of GPI-anchored proteins before arrival to the Golgi. The end8-1/lcb1-100 mutant is defective in ceramide synthesis. In vitro data suggest a requirement for ceramides after the exit from the ER. We therefore propose that ceramides might function in the fusion of a GPI-containing vesicle with the Golgi, but we cannot exclude a role in the ER. The second mutant that blocks the transport of GPI-anchored proteins to the Golgi is ret1-1, a mutant in the α-subunit of coatomer. In both mutants, GPI-anchor attachment is normal and in ret1-1 cells, the GPI-anchors are remodeled with ceramide to the same extent as in wild-type cells.

Original languageEnglish
Pages (from-to)2703-2714
Number of pages12
JournalJournal of cell science
Volume110
Issue number21
StatePublished - Dec 3 1997
Externally publishedYes

Keywords

  • Biosynthetic transport
  • COP II
  • Ceramide
  • Coatomer
  • Glycosylphosphatidylinositol

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    Sütterlin, C., Doering, T. L., Schimmöller, F., Schröder, S., & Riezman, H. (1997). Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast. Journal of cell science, 110(21), 2703-2714.