@article{a4852e39c8364f2eae6540d86682d021,
title = "Specific recruitment of SH-PTP1 to the erythropoietin receptor causes inactivation of JAK2 and termination of proliferative signals",
abstract = "The binding of erythropoietin (EPO) to its receptor (EPO-R) activates the protein tyrosine kinase JAK2. The mechanism of JAK2 inactivation has been unclear. We show that the hematopoietic protein tyrosine phosphatase SH-PTP1 (also called HCP and PTPIC) associates via its SH2 domains with the tyrosine-phosphorylated EPO-R. In vitro binding studies suggest that Y429 in the cytoplasmic domain of the EPO-R is the binding site for SH-PTP1. Mutant EPO-Rs lacking Y429 are unable to bind SH-PTP1; cells expressing such mutants are hypersensitive to EPO and display prolonged EPO-induced autophosphorylation of JAK2. Our results suggest that activation of SH-PTP1 by binding to the EPO-R plays a major role in terminating proliferative signals.",
author = "Ursula Klingm{\"u}ller and Ulrike Lorenz and Cantley, {Lewis C.} and Neel, {Benjamin G.} and Lodish, {Harvey F.}",
note = "Funding Information: Correspondence for this work should be addressed to H. F. L. This work was supported by grant HL32262 from the National Institutes of Health, a grant from the Arris Pharmaceutical Corporation (H. F. L.), and a grant from the Lucille P. Markey Charitable Trust (L. C. C.). B. G. N. is the recipient of a Junior Faculty Research Award from the American Cancer Society and the Gertrude Elion Award from the American Association of Cancer Research, funded by the Burroughs-We,come Corporationis, and he is supported by funds from the Harvard Medical School/F. Hoffmann-LaRoche Institute for Chemistry and Medicine. U. K. was supported by a fellowship from the Deutsche Forschungsgemeinschaft, and U. L. is supported by a fellowship from the American Cancer Society, Massachusetts Division. We thank Drs. F. U. Reuss, K. Luo, and S. Michnick for many helpful discussions and for critically reading the manuscript. We also thank Dr. K. Ravi-chandran for the help with the proliferation assays and Dr. B. Druker for the generous gifts of the anti-PTyr monoclonal antibody 4G10.",
year = "1995",
month = mar,
day = "10",
doi = "10.1016/0092-8674(95)90351-8",
language = "English",
volume = "80",
pages = "729--738",
journal = "Cell",
issn = "0092-8674",
number = "5",
}