Specific Immunofluorescence Test for Detection of Herpesvirus Antigen in Cells of the Lucke Renal Adenocarcinoma

Steven M. Paul; Merle Mizell; Branch Craige; Julia Blazek; Margaret Skinner

doi:10.3181/00379727-139-36272

Specific Immunofluorescence Test for Detection of Herpesvirus Antigen in Cells of the Lucke Renal Adenocarcinoma

Steven M. Paul, Merle Mizell, Branch Craige, Julia Blazek, Margaret Skinner

Washington University School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Antiserum prepared against purified herpesvirus fractions of the Lucké tumor has been used in detecting the virus-containing nature of various tumor samples. Bright fluorescence of winter tumor cells was observed when reacted in the indirect immunofluorescence test with the prepared antiserum. No staining was observed in tumor samples not harboring the herpesvirus or in normal frog kidney sections. The evidence that the antiserum is specific for a frog herpesvirus antigen(s) is discussed.

Original language	English
Pages (from-to)	944-948
Number of pages	5
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	139
Issue number	3
DOIs	https://doi.org/10.3181/00379727-139-36272
State	Published - Mar 1972

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title = "Specific Immunofluorescence Test for Detection of Herpesvirus Antigen in Cells of the Lucke Renal Adenocarcinoma",

abstract = "Antiserum prepared against purified herpesvirus fractions of the Luck{\'e} tumor has been used in detecting the virus-containing nature of various tumor samples. Bright fluorescence of winter tumor cells was observed when reacted in the indirect immunofluorescence test with the prepared antiserum. No staining was observed in tumor samples not harboring the herpesvirus or in normal frog kidney sections. The evidence that the antiserum is specific for a frog herpesvirus antigen(s) is discussed.",

author = "Paul, {Steven M.} and Merle Mizell and Branch Craige and Julia Blazek and Margaret Skinner",

note = "Funding Information: These investigations were supported by U.S. Public Health Service Grant CA-011901 from the National Cancer Institute, USPHS − 5 SO1-FRR-05377-11 and Damon Runyan Grant DRG-1055. 2 ",

year = "1972",

month = mar,

doi = "10.3181/00379727-139-36272",

language = "English",

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T1 - Specific Immunofluorescence Test for Detection of Herpesvirus Antigen in Cells of the Lucke Renal Adenocarcinoma

AU - Paul, Steven M.

AU - Mizell, Merle

AU - Craige, Branch

AU - Blazek, Julia

AU - Skinner, Margaret

N1 - Funding Information: These investigations were supported by U.S. Public Health Service Grant CA-011901 from the National Cancer Institute, USPHS − 5 SO1-FRR-05377-11 and Damon Runyan Grant DRG-1055. 2

PY - 1972/3

Y1 - 1972/3

N2 - Antiserum prepared against purified herpesvirus fractions of the Lucké tumor has been used in detecting the virus-containing nature of various tumor samples. Bright fluorescence of winter tumor cells was observed when reacted in the indirect immunofluorescence test with the prepared antiserum. No staining was observed in tumor samples not harboring the herpesvirus or in normal frog kidney sections. The evidence that the antiserum is specific for a frog herpesvirus antigen(s) is discussed.

AB - Antiserum prepared against purified herpesvirus fractions of the Lucké tumor has been used in detecting the virus-containing nature of various tumor samples. Bright fluorescence of winter tumor cells was observed when reacted in the indirect immunofluorescence test with the prepared antiserum. No staining was observed in tumor samples not harboring the herpesvirus or in normal frog kidney sections. The evidence that the antiserum is specific for a frog herpesvirus antigen(s) is discussed.

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JO - Proceedings of the Society for Experimental Biology and Medicine

JF - Proceedings of the Society for Experimental Biology and Medicine

IS - 3

ER -

Specific Immunofluorescence Test for Detection of Herpesvirus Antigen in Cells of the Lucke Renal Adenocarcinoma

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