Specific DNA sequences associated with the nuclear matrix in synchronized mouse 3T3 cells

G. I. Goldberg, I. Collier, A. Cassel

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Eukaryotic chromatin appears to be organized into arrays of supercoiled loops anchored to the scaffolding structure of the mitotic chromosome core or to the nuclear matrix of interphase nuclei. To reveal whether specific DNA sequences are involved in this level of chromatin organization, we isolated and cloned a population of DNA molecules [averaged length of 150 base pairs (bp)] closely associated with the nuclear matrix after exhaustive DNase digestion and subsequent extensive protease digestion. The nuclear matrix was obtained from murine BALB/c 3T3 cells synchronized at the G1/S border of the cell cycle. We report the structure of two sequences, designated G4 and G5, which are highly enriched in the matrix DNA. Sequence G4, of 152 bp, contains three 31-bp direct ehat-to-tail repeats. An 11-bp sequence at the end of each repeat is homologous to the first large tumor antigen recognition site of human papova virus. Sequence G5, of 135 bp, consists of two well-defined domains, in which the first domain is a fragment of the B1 repetitive sequence. The results suggest the possibility that the loops of histone-depleted chromatin are connected to the scaffold of the nuclear matrix, with sspecific DNA sequences at the anchorage sites.

Original languageEnglish
Pages (from-to)6887-6891
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number22 I
DOIs
StatePublished - Jan 1 1983

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