Sonoporation of cells for drug and gene delivery

H. Pan, Y. Zhou, F. Sieling, J. Shi, J. Cui, C. Deng

Research output: Contribution to journalConference articlepeer-review

12 Scopus citations

Abstract

Recent studies demonstrate that ultrasound can be used to deliver compounds into viable cells for potential targeted drug delivery and non-viral gene transfection, revealing new, advantageous possibilities. The delivery is facilitated through sonoporation, the formation of temporary pores in the cell membrane induced by ultrasound. Our study focuses on the study of sonoporation mechanisms in order to achieve optimal delivery outcome such as high delivery efficiency and minimal cell death. Using voltage clamp techniques, we obtained real-time measurements of sonoporation of single Xenopus oocytes in the presence of Optison™, an agent consisting of albumin-shelled C3F 8 gas bubbles. Ultrasound increased the transmembrane current as a direct result of decreased membrane resistance due to pore formation. The ability to real time monitor sonoporation of cells provides a novel and necessary tool for us to study the dynamic sonoporation process and obtain optimal delivery parameters. We confirmed the delivery of compound into cells by using markers such as plasmid GFP.

Original languageEnglish
Pages (from-to)3531-3534
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume26 V
StatePublished - 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: Sep 1 2004Sep 5 2004

Keywords

  • Cell porosity
  • Cells, cell membrane
  • Drug delivery
  • Gene transfection
  • Sonoporation
  • Ultrasound

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