TY - JOUR
T1 - Somatostatin and its receptors
T2 - Functional regulation in the development of mice Sertoli cells
AU - Riaz, Hasan
AU - Liang, Aixin
AU - Khan, Muhammad Kasib
AU - Dong, Ping
AU - Han, Li
AU - Shahzad, Muhammad
AU - Chong, Zhenlu
AU - Ahmad, Sibtain
AU - Hua, Guohua
AU - Yang, Liguo
N1 - Funding Information:
This paper was supported by both the earmarked funds for Modern Agro-industry Technology Research System ( CARS-37-04B ) and International Cooperation key Project of China ( OS2012ZR0216 ).
PY - 2013
Y1 - 2013
N2 - Recently, Sertoli cells have been ascertained as the target for the regulatory peptide somatostatin (SST). Therefore, the present study investigated the expression of somatostatin receptors, their age-related alterations and homologous regulation by in vitro treatment with SRIF14 on mice Sertoli cells; furthermore, it dealt with SRIF14 action on growth progression, apoptotic activity and related gene expressions in these cells. We found that mice Sertoli cells expressed all SST1-5 receptors with differential intensities. Age-related real-time PCR of all somatostatin receptors identified abundance of SSTR2 and SSTR5 mRNA level during Sertoli cell developmental period. Furthermore, higher level of these two receptors was independent of SRIF14, as treatment with SRIF14 failed to induce both receptor expressions when compared with control. Somatostatin treatment elicited a dose-dependent decrease in forskolin stimulated cAMP production. Low (100 pM and 10 nM) and high dosage (1 μM) groups of SRIF14 significantly promoted apoptosis, while all treatment groups led to dose dependent cessation (P < 0.05) of G1 phase of cell cycle as further validated by increase in casp3, decrease in bcl2, elevation of P21 (all by western blot) and decrease in Igf1 expressions, similarly, SST treatment caused a dose dependent suppression in the mRNA level of kitl gene, which is important in the regulation of spermatogenesis. These findings suggest that somatostatin and its receptors (SSTR2 and SSTR5) are important markers in the regulation and development of Sertoli cell; furthermore, it portrays physiological inhibitory role in Sertoli cell development by inducing apoptosis and cell cycle arrest.
AB - Recently, Sertoli cells have been ascertained as the target for the regulatory peptide somatostatin (SST). Therefore, the present study investigated the expression of somatostatin receptors, their age-related alterations and homologous regulation by in vitro treatment with SRIF14 on mice Sertoli cells; furthermore, it dealt with SRIF14 action on growth progression, apoptotic activity and related gene expressions in these cells. We found that mice Sertoli cells expressed all SST1-5 receptors with differential intensities. Age-related real-time PCR of all somatostatin receptors identified abundance of SSTR2 and SSTR5 mRNA level during Sertoli cell developmental period. Furthermore, higher level of these two receptors was independent of SRIF14, as treatment with SRIF14 failed to induce both receptor expressions when compared with control. Somatostatin treatment elicited a dose-dependent decrease in forskolin stimulated cAMP production. Low (100 pM and 10 nM) and high dosage (1 μM) groups of SRIF14 significantly promoted apoptosis, while all treatment groups led to dose dependent cessation (P < 0.05) of G1 phase of cell cycle as further validated by increase in casp3, decrease in bcl2, elevation of P21 (all by western blot) and decrease in Igf1 expressions, similarly, SST treatment caused a dose dependent suppression in the mRNA level of kitl gene, which is important in the regulation of spermatogenesis. These findings suggest that somatostatin and its receptors (SSTR2 and SSTR5) are important markers in the regulation and development of Sertoli cell; furthermore, it portrays physiological inhibitory role in Sertoli cell development by inducing apoptosis and cell cycle arrest.
KW - Apoptosis
KW - Cell cycle
KW - Sertoli cells
KW - Somatostatin
KW - cAMP
UR - http://www.scopus.com/inward/record.url?scp=84884544662&partnerID=8YFLogxK
U2 - 10.1016/j.jsbmb.2013.06.007
DO - 10.1016/j.jsbmb.2013.06.007
M3 - Article
C2 - 23831358
AN - SCOPUS:84884544662
SN - 0960-0760
VL - 138
SP - 257
EP - 266
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
ER -