Somatic mutations and clonal hematopoiesis in congenital neutropenia

Jun Xia, Christopher Miller, Jack Baty, Amrita Ramesh, Matthew R.M. Jotte, Robert Fulton, Tiphanie P. Vogel, Megan Cooper, Kelly J. Walkovich, Vahagn Makaryan, Audrey A. Bolyard, Mary Dinauer, David Wilson, Adrianna Vlachos, Kasiani C. Myers, Robert J. Rothbaum, Alison A. Bertuch, David C. Dale, Akiko Shimamura, Laurence A. BoxerDaniel Link

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Severe congenital neutropenia (SCN) and Shwachman-Diamond syndrome (SDS) are congenital neutropenia syndromes with a high rate of leukemic transformation. Hematopoietic stressors may contribute to leukemic transformation by increasing the mutation rate in hematopoietic stem/progenitor cells (HSPCs) and/or by promoting clonal hematopoiesis. We sequenced the exome of individual hematopoietic colonies derived from 13 patients with congenital neutropenia to measure total mutation burden and performed error-corrected sequencing on a panel of 46 genes on 80 patients with congenital neutropenia to assess for clonal hematopoiesis. An average of 3.6 ± 1.2 somatic mutations per exome was identified in HSPCs from patients with SCN compared with 3.960.4 for healthy controls (P = NS). Clonal hematopoiesis due to mutations in TP53 was present in 48% (13/ 27) of patients with SDS but was not seen in healthy controls (0/17, P < .001) or patients with SCN (0/40, P < .001). Our SDS cohort was young (median age 6.3 years), and many of the patients had multiple TP53 mutations. Conversely, clonal hematopoiesis due to mutations of CSF3R was present in patients with SCN but was not detected in healthy controls or patients with SDS. These data show that hematopoietic stress, including granulocyte colony-stimulating factor, do not increase the mutation burden in HSPCs in congenital neutropenia. Rather, distinct hematopoietic stressors result in the selective expansion of HSPCs carrying specific gene mutations. In particular, in SDS there is enormous selective pressure to expand TP53-mutated HSPCs, suggesting that acquisition of TP53 mutations is an early, likely initiating event, in the transformation to myelodysplastic syndrome/acute myeloid leukemia in patients with SDS.

Original languageEnglish
Pages (from-to)408-416
Number of pages9
JournalBlood
Volume131
Issue number4
DOIs
StatePublished - Jan 25 2018

Fingerprint Dive into the research topics of 'Somatic mutations and clonal hematopoiesis in congenital neutropenia'. Together they form a unique fingerprint.

  • Cite this

    Xia, J., Miller, C., Baty, J., Ramesh, A., Jotte, M. R. M., Fulton, R., Vogel, T. P., Cooper, M., Walkovich, K. J., Makaryan, V., Bolyard, A. A., Dinauer, M., Wilson, D., Vlachos, A., Myers, K. C., Rothbaum, R. J., Bertuch, A. A., Dale, D. C., Shimamura, A., ... Link, D. (2018). Somatic mutations and clonal hematopoiesis in congenital neutropenia. Blood, 131(4), 408-416. https://doi.org/10.1182/blood-2017-08-801985